摘要
硫酸盐还原菌(Sulfate-reducing bacteria,SRB)是油田回注污水中的重要细菌,会引起腐蚀和堵塞问题.在油田回注污水中加入碱,将pH值从6.5-7.5升高到7.6-8.2,SRB总体数量浓度明显降低,为了了解碱对污水中的SRB群落结构的影响,本研究应用SRB功能基因分析方法,构建污水中硫酸盐还原菌亚硫酸盐异化酶基因(dsr)系统发育树,探讨加碱前后回注污水中SRB群落结构的变化.结果发现在没加碱之前,污水中存在SRB高达15个属,而长期加碱改变其pH值后,污水中仅存6个属,分别是脱硫弧菌属(Desulfovibrio)、脱硫状菌属(Desulfacinum)、脱硫杆菌属(Desulfobacerium)、脱硫念珠菌属(Desulfomonile)、脱硫微菌属(Desulfomicrobium)和脱硫盐菌属(Desulfohalobium)等,另外还新出现两个属,分别是脱硫叶菌属(Desulfobulbus)和脱硫豆菌属(Desulfotignum).说明加碱处理改变了污水中的SRB生态结构,很多SRB种类不能适应碱性环境,这8个属的SRB具有耐碱性,现场污水的腐蚀性测试结果表明,碱性环境不仅减少了污水中的SRB种类,同时还抑制了幸存的SRB的活性,控制了污水的腐蚀速率.
Sulfate-reducing bacteria（SRB）, important bacteria in oilfield rejection water, often cause corrosion and formation damage. Addition of alkali in oilfield rejection water can significantly decrease the overall concentration of SRB by elevating the solution pH from 6.5–7.5 to 7.6–8.2. This work constructed the phylogenetic tree from dissimilatory sulfite reductase genes of SRB and investigated the effect of alkali addition on the community structure of SRB in rejection water by analyzing their function genes. The results showed that only 6 genera（desulfovibrio, desulfacinum, desulfobacerium, desulfomonile, desulfomicrobium, and desulfohalobium） were detected after long-term alkali addition, in strong contrast to the number of genera（15） determined in the oilfield reinjection water before treatment. Among the preserved alkali-resistant genera, desulfovibrio and desulfacinum were two new genera found in the solution after alkali treatment. Our study indicated that most SRB could not adapt to the alkaline environment, which changed the community structure of SRB. The field erosion test suggested that addition of alkali not only reduces the SRB species, but also inhibits the activity of the surviving SRB, in general helps controlling the corrosion rate.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2015年第6期1055-1059,共5页
Chinese Journal of Applied and Environmental Biology
基金
中石化胜利油田科研项目(YKS1206)资助~~
关键词
油田回注污水
硫酸盐还原菌
耐碱性
腐蚀率
oilfield reinjection water
sulfate-reducing bacteria
alkali-resistant
corrosion rate
