摘要
2014年在广东辣椒产区发现疑似番茄斑萎病毒属(Tospovirus)病毒侵染引起的辣椒褪绿坏死环斑症状。Dot-ELISA检测显示,该病毒分离物与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)单克隆抗体不产生血清学反应,表明该病毒不属于TSWV。利用Tospovirus属病毒通用引物gL3637/gL4435C进行RT-PCR检测,可以从所有辣椒病样总RNA中扩增出预期大小约800 bp的目的片段。基因克隆与序列分析表明,该片段序列与西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)中国广州分离物的同源性最高,为97.5%。系统进化分析也显示,该病毒分离物与WSMoV各分离物亲缘关系最近,并聚类在一个分支。因此,侵染广东辣椒的病毒分离物为WSMoV。
Pepper plants showing chlorotic and necrotic rings symptoms on the leaves reminiscent of infection by tospoviruses were found in pepper-production fields of Guangdong in 2014.The infected samples were detected by Dot-ELISA and RT-PCR,respectively.Negative results were obtained in the Dot-ELISA with monoclonal antibody of Tomato spotted wilt virus（TSWV）.This result indicated that the virus isolate didn＇t belong to TSWV.However,an expected approximately 800 bp-fragment were obtained from all infected pepper samples by RT-PCR using the universal primer pair gL3637/gL4435 C for tospo viruses.The results of gene cloning and sequence analysis showed that the fragment shared the highest identity at 97.5% with corresponding parts of the Guangzhou isolate of Watermelon silver mottle virus（WSMoV）.Phylogenic analysis showed that the virus isolate infecting pepper in Guangdong is most closely related to the reported isolates of WSMoV,and that they clustered in a monophyletic group.Therefore,the virus isolate infecting pepper in Guangdong is an isolate of WSMoV.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第11期2261-2266,共6页
Acta Horticulturae Sinica
基金
国家公益性行业(农业)科研专项(201303028)
广东省科技计划项目(2013B020309003)
关键词
辣椒
西瓜银斑驳病毒
分子鉴定
pepper
Watermelon silver mottle virus
molecular identification
