摘要
在云南省临沧市永德县海拔约800m的澳洲坚果(Macadamia sp.)种苗繁育基地发现叶片褪绿、黄化、叶脉间呈现近似圆形或不规则褐色枯斑的植株。为了确认植株病因,采集典型病株,应用电子显微镜负染色法、超薄切片和DAS-ELISA进行检测,在澳洲坚果苗木病叶分离物Maca-LC的粗汁液和病叶超薄切片中均观察到有类似番茄斑萎病毒属(Tospovirus)病毒粒体。该病毒粒子为球形,直径80～85nm。进一步应用DAS-ELISA方法鉴定,病叶与Tospovirus的西瓜银色斑驳病毒(Watermelon silver mottle virus,WSMoV)/花生芽坏死病毒(Groundnut bud necrosisvirus,GBNV)复合抗血清呈阳性反应。通过病症典型症状、病毒形态学观察及血清学方法检测表明,侵染云南澳洲坚果种苗的病毒分离物Maca-LC为番茄斑萎病毒属Tospovirus病毒。
Infected plant seedlings of macadamia showing concentric chlorotic ring spot or brown lethal spot symptoms were found in a nursing base located at the altitude of about 800 meters in Lincang, Yunnan, China. In order to confirm the presence of virus, typically infected leaves of Maeadamia sp. were detected by using electron microscopy and DAS-ELISA. Virions with a diameter of about 80 - 85 rim, similar in size to the Tospovirus particles, were both observed in the sap and ultrathin sections of diseased leaves of macadamia. The crude extracts of infected leaves had a positive reaction to the complex antibody of Watermelon silver mottle virus （ WSMoV ） /Groundnut bud necrosis virus （ GBNV ） . These results above indicated that macadamia was infected by a Tospovirus. It is the first report of Tospovirus infecting macadamia.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第2期350-354,共5页
Acta Horticulturae Sinica
基金
云南省创新团队计划项目(2011C1134)
云南省中青年学术技术带头人项目(2008PY0S0)
国家自然科学基金项目(31060237)
