摘要
作者应用鼠伤寒沙门氏菌回变试验、小鼠骨髓细胞微核试验及CHO细胞染色畸变试验,对国产氨利酮与甲腈吡酮进行诱变性试验研究。结果:氨利酮和甲腈吡酮在鼠伤寒沙门氏菌回变试验均为阴性,CHO细胞染色体畸变试验均为阳性;氨利酮剂量为其临床有效剂量的70～210倍时,小鼠骨髓细胞微核试验为阳性,甲腈吡酮则为阴性。提示该两药均不引起基因突变,但氨利酮可能在体内、外引起染色体损伤,甲腈吡酮在体外引起染色体损伤,可能通过体内代谢变弱,故在诱变性方面甲腈吡酮可能比氨利酮更为安全。
The mutagenicity of domestic new drugsamrinone and milrinone were studied byAmes test, micronucleus test of mouse bonemarrow and chromosome aberration assayin CHO cells. The results were as follows: neitheramrinone nor milrinone was mutagenic inthe Ames test; in chromosome aberrationassay, both gave positive results; in mousemicroncleus test, amrinone gave a positiveresult when mice were exposed to 0.8 LD_(??)dose, but milrinone gave a negativeresult. These results suggested that amrinoneand milrinone did not induce gene muta-tion, but amrinone induced chromosomedamage both in vitro and in vivo, while thechromosome-damaging activity of milrinonein vitro may be minimized by biodatoxica-tion in vivo.
出处
《华西医科大学学报》
CAS
CSCD
1989年第2期181-185,共5页
Journal of West China University of Medical Sciences
关键词
AMES试验
微核
试验
氨利酮
诱变
Ames test
Micronucleus test
Chomosome aberration assay Amrinone
Milrinone
