摘要
目的:研究银杏二萜内酯葡胺注射液与人血浆蛋白的结合情况。方法:采用平衡透析法测定银杏二萜内酯葡胺注射液中银杏内酯A(GA)、银杏内酯B(GB)和银杏内酯K(GK)与人血浆蛋白的结合率,以LC-MS/MS法测定透析内外液中各待测物的浓度,计算其血浆蛋白结合率。结果:血浆中其他成分对测定无干扰,各待测物在选择浓度范围内线性关系均良好,精密度及稳定性结果均符合方法学要求;样品孵育8 h后,GA、GB和GK与人血浆蛋白结合基本达到平衡,此时GA(0.34、1.70和8.51μg·m L-1)与人血浆蛋白结合率为84.03%-88.11%,GB(0.62、3.09和15.5μg·m L^(-1))与人血浆蛋白结合率为41.21%-53.56%,GK(0.04、0.20和1.01μg·m L-1)与人血浆蛋白结合率为45.24%-59.59%。结论:该方法操作简便、快速,灵敏度高,能满足生物样品的分析要求。GA与血浆蛋白结合较强,GB和GK具有中等强度的蛋白结合率。
This paper was aimed to study the human plasma protein binding rate of diterpene ginkgolides meglumine injection. The equilibrium dialysis was used to determine the human plasma protein binding rate of ginkgolide A（GA）, ginkgolide B（GB） and ginkgolide K（GK） in diterpene ginkgolides meglumine injection. The LC-MS/MS method was used for the content determination of ginkgolides. And then, the plasma protein binding rate was calculated. The results showed that there was no interference from other ingredients for the determination of ginkgolides. The calibration curve of the analytes was in good linearity in certain range of contents. The precision and stability of the analytes met the methodology requirements. After 8 h incubation, the human plasma protein binding rate of GA, GB and GK achieved balance. The human plasma protein binding rate of GA（0.34, 1.70 and 8.51 μg·m L-1） was 84.03%-88.11%; the human plasma protein binding rate of GB（0.62, 3.09 and 15.5 μg·m L-1） was 41.21%-53.56%; the human plasma protein binding rate of GK（0.04, 0.20 and 1.01 μg·m L-1） was 45.24%-59.59%. It was concluded that the method was simple, rapid and sensitive, which met the analysis requirement for biological samples. GA had a high plasma protein binding rate; GB and GK had medium plasma protein binding rate.
出处
《世界科学技术-中医药现代化》
2015年第11期2235-2239,共5页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
科学技术部国家重大新药创制专项(2013ZX09402203):现代中药创新集群与数字制药技术平台
负责人:王振中
