摘要
目的:研究白头翁皂苷D与不同血浆蛋白的结合率。方法:采用HPLC与平衡透析法相结合的方法测定白头翁皂苷D与牛血清、人血浆及大鼠血浆中蛋白的结合率,COSMOSIL 5C18-MS-Ⅱ色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水-甲酸(80∶20∶0.02),检测波长203 nm。结果:白头翁皂苷D质量浓度为0.06,0.11,0.25 g·L-1时与牛血清白蛋白的结合率分别为(78.45±0.89)%,(77.61±1.14)%,(77.16±0.29)%,与人血浆蛋白的结合率分别为(68.91±0.54)%,(67.68±0.87)%,(67.88±0.71)%,与大鼠血浆蛋白的结合率分别为(78.15±0.76)%,(78.61±0.97)%,(78.24±0.93)%。结论:建立的白头翁皂苷D蛋白结合率测定方法简便、稳定、可靠。白头翁皂苷D是一种中等程度的蛋白结合药物,结合率不具有质量浓度依赖性。
Objective：To investigate different plasma protein binding rates of pulsatilla saponin D.Method：HPLC and equilibrium dialysis method were adopted to determine plasma protein binding rates of pulsatilla saponin D in bovine serum,human and rat plasma,COSMOSIL 5C18-MS-Ⅱ column （4.6 mm × 250 mm,5 μm） was employed with mobile phase of methanol-water-formic acid （80∶ 20∶ 0.02） and detection wavelength at 203 nm.Result：Plasma protein binding rates of pulsatilla saponin D at three various concentrations （0.06,0.11,0.25 g·L-1） were （78.45±0.89）％,（77.61±1.14）％,（77.16±0.29）％ in bovine serum albumin,they were （68.91±0.54）％,（67.68±0.87）％,（67.88±0.71）％ in human plasma and （78.15 ±0.76）％,（78.61 ±0.97）％,（78.24 ±0.93）％ in rat plasma,respectively.Conclusion：This method for determining plasma protein binding rates of pulsatilla saponin D was simple,stable and reliable.Pulsatilla saponin D was a drug with moderate plasma protein binding rate,which did not have concentration-dependent manner.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期114-117,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2011ZX11102-001-19)
江西省自然科学基金项目(20114BAB215045)
江西省优势科技创新团队计划项目(2010DQB01700)
江西省教育厅产学研合作项目(GJJ11006)
