摘要
目的:探讨基质金属蛋白酶-9(MMP-9)在急性胰腺炎相关性肺损伤(APALI)中的表达及其临床意义。方法:SD大鼠腹腔注射L-精氨酸制备急性坏死性胰腺炎(ANP)模型。透射电镜下观察大鼠肺组织超微结构改变;采用免疫组化法分析MMP-9在大鼠肺脏、胰腺组织中的表达及定位。对ANP死亡患者尸检的肺脏和胰腺标本蜡块进行HE染色和免疫组化分析。结果:透射电镜观察ANP大鼠肺泡基底膜水肿、坏死、断裂,肺脏间质水肿、坏死、大量粒细胞浸润,毛细血管内皮水肿、坏死、断裂;免疫组化显示死亡大鼠肺间质、基底膜及间质浸润的炎症细胞和胰腺间质、基底膜MMP-9均表达阳性。ANP尸检标本胰腺和肺脏组织有明显的坏死和结构破坏,且MMP-9表达阳性。结论:MMP-9在ANP大鼠和死亡患者胰腺和肺脏组织表达均阳性,MMP-9可能在APALI的发病过程中起着重要作用。
Objective： To investigate the expression of matrix metalloproteinase-9（ MMP-9） in acute pancreatitis associated lung injury（ APALI） and its clinical significance. Methods： Models of acute necrotizing pancreatitis（ ANP） were established in SD rats by intraperitoneal injection of L-arginine. The Ultra-structural changes of lung were observed under transmission electron microscope,and the expression and location of MMP-9 in lung and pancreas were analyzed by immunohistochemistry. Human ANP lung and pancreas tissues obtained from autopsy specimen also underwent HE staining and immunohistochemical analysis. Results： The ANP rat showed ultramicroscopically edema,necrosis,fragmentation of pulmonary alveoli basement membrane; pulmonary interstitial edema,necrosis,massive granulocytes infiltration; edema and necrosis of capillary endothelium. Immunohistochemical examination demonstrated positive MMP-9 expression on the inflammatory cells of basement membrane and stroma of lung and pancreas. ANP autopsy specimens of pancreas and lung tissue also showed obvious necrosis and structural damage,and MMP-9 expression was positive. Conclusions： MMP-9 expression of lung and pancreas tissues in ANP rats and patients was positive,and MMP-9 may play an important role in the pathogenesis of APALI.
出处
《内科急危重症杂志》
2015年第6期452-455,共4页
Journal of Critical Care In Internal Medicine
基金
上海市卫生系统百名跨世纪优秀学科带头人培养计划(No:98BR026)
关键词
基质金属蛋白酶
胰腺炎
肺损伤
Matrix metalloproteinase
Pancreatitis
Lung injury
