摘要
作者提取不同群、型和毒力的构端螺旋体糖脂蛋白,以Rhodamine B荧光素和胶体金标记制备成探针,与体外培养的牛主动脉内皮细胞作用,以荧光显微镜和扫描电镜观察记录其过程,并以内皮细胞LDH、AcP漏出率和蛋白含量及细胞形态学作为细胞毒指标。结果表明,钧端螺旋体糖脂蛋白通过对培养血管内皮细胞的粘附,导致细胞内吞与病损。该过程可能属于一种特殊的受体介导内吞过程。
The Glycolipoprotein (GLP) of variousvirulent leptospires was extracted, purifiedand identified. The GLP was combined withfiuorescence rhodamine B and the colloidalgold as the probe. Meanwhile, the bovineaortic endothelial cell (EN) was isolated andcultured in the medium 199 'Nissui' . TheGLP-rhodamine B and GLP-colloidal goldprobe were added to cultured EN and incu-bated at 4℃ for 2 h then 37℃ for 2 h. Theinteraction between GLP-rhodamine B,GLP-CG probe and EN were observed andphotographied by fluorescence microscopy(Leitz) and scanning electromicroscopy. Thereleasing rate of lactic acid dehydrogenase(LDH), acid phosphatase (AcP) and proteinfrom the EN were detected. The results in-dicated that the GLP of Leptospira ihterro-gans serovar Lai, strain 017 attached to ENand a endocytosis was happened subsequent-ly. The LDH and AcP releasing rate weresignificantly higher than control and thecell protein was much less than control. Theresults showed that the GLP of L. interro-gans strain 017 had the cytotoxin effectthrough a specific attachment, endoeytosisin EN.The endocytosis probably is a recep-tor-mediated endocytosis.
出处
《华西医科大学学报》
CAS
CSCD
1989年第2期115-118,共4页
Journal of West China University of Medical Sciences
基金
卫生部科研基金
关键词
钩端螺旋体
糖脂蛋白
内皮细胞
Leptospira
Glycolipoprotein
Endothelial cell in culture
Probe Receptor-mediated endocytosis
