期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

致病钩端螺旋体特异消减片段的序列测定与分析 被引量：1

Sequencing and Analysis of the Genomic Subtracted Fragments between the Pathogenic Leptospira and Nonpathogenic Leptospira by SSH
暂未订购
导出
摘要 对24个已鉴定的赖型致病钩端螺旋体特异消减片段进行DNA序列测定和生物信息学分析.结果显示,片段两端皆含有AluⅠ酶切位点,片段大小为149～1506bp,平均480bp,GC含量平均为36 63%.其中6个序列无任何同源匹配序列,提示可能来自新基因;18个同源序列可分为两类:与外膜蛋白或假想蛋白相关,与生化代谢修饰相关的酶.在Genbank中登记其中20个序列,序列号分别为AF300873-AF300877,AF325807-AF325821.这些基于生物信息学的功能预测,将有助于在此基础上进行相关的基因克隆鉴定、表达分析及功能验证. 24 subtracted fragments between leptospira seravor lai and biflex seravor Patoc Ⅰby SSH methods were sequenced, and analyzed with the bioinformatics tools such as Omiga 2.0, Athenport, SAPS, SignalP. The results showed the sequence size was from 149 bp to 1506 bp average of 480 bp, the GC content was 36.63%. 6 of all sequences were no homological they may came from the new gene; 18 which had some homologies were divided into two classes, one was homologous to membrane protein or hypothetical protein, the other was homological with some enzymes. And 20 of these sequences were deposited in Genbank with the Noumbers AF300873-AF300877, AF325807-AF325821. These results based on the bioinformatics predication will be helpful to clone and validate related specific genes in the pathogenic leptospira.
作者 胡昌华 鲍朗 毕小君
机构地区 西南师范大学现代生物医药研究所生命科学学院 四川大学华西医学中心
出处 《西南师范大学学报（自然科学版）》 CAS CSCD 北大核心 2004年第3期434-438,共5页 Journal of Southwest China Normal University(Natural Science Edition)
基金 国家自然科学基金资助项目(30070670) 重庆市应用基础研究资助项目(2002-7481).
关键词 钩端螺旋体 差异消减片段 序列测定 生物信息学 leptospira subtracted fragments sequencing bioinformatics
分类号 R377.5 [医药卫生—病原生物学]
  • 相关文献

参考文献11

  • 1Faine S. Leptospirosis [ A ]. In Bacterial Infections of Humans, 3 rd edited by Evans A and Brachman P [ C ]. New York: Plenum Medical Book Company. 1998. 395- 419.
  • 2Zuerner R L, Herrmann J L, Girons I S. Comparison of Genetic Maps for two Leptospira Interrogans Serovars Provides Evidence for two Chromosomes and Intraspecies Heterogeneity [J]. J Bacteriol, 1993, 175(17): 5445- 5451.
  • 3Faraldo M M, Pedro M A, Berenguer J. Sequence of the S-layer Gene of Thermus Thermophilus HB8 and Functionality of Its Promoter in E. coli [J]. J Bacterial, 1992, 174(22): 7458 - 7462.
  • 4Nelson K E, Clayton R A, Gill S R, et al. Evidence for Lateral Gene Transfer Between Arehaea and Bacteria from Genome Sequence of Thermotoga Maritime [J]. Nature, 1999, 399(6734): 323 - 329.
  • 5Raikhel N, Somerville S, Thomashow M, et al. Genes Galore: a Summary of Methods for Accessing Results from Large-Scale Partial Sequencing of Anonymous Arabidopsis cDNA Clones [J]. Plant Physiol, 1994, 106(4): 1241 - 55.
  • 6Sara M, Sleytr U B. S-layer proteins[J]. J Bacteriol, 2000 182(4): 859- 868.
  • 7Haker J, Kaper J B. Pathogenicity Islands and the Evolution of Microbes [J]. Annu Rev Microbiol, 2000, 54:641 - 679.
  • 8Parkhill J, Achtman M, James K D, et al. Complete DNA Sequence of a Serogroup A Strain of Neisseria Meningitidis Z2491 [ J]. Nature, 2000; 404(6777): 502- 506.
  • 9Ratledge C, Dover L. Iron Metabolism in Pathogenic Bacteria [J]. Annu Rew Microbiol, 2000, 54:881 -941.
  • 10鲍朗,胡昌华,李学敏,晏菊芳,张万江,张会东.利用抑制消减杂交技术研究钩端螺旋体致病相关基因[J].中华微生物学和免疫学杂志,2001,21(5):574-577. 被引量：12

二级参考文献6

  • 1Haake D A，Infect Immun，2000年，68卷，4期，2276页
  • 2Zhang Y L，Microbiology，2000年，146卷，4期，999页
  • 3Alm R A，Nature，1999年，397卷，176页
  • 4Diatchenko L，Proc Nat Acad Sci USA，1996年，93卷，6025页
  • 5Lynn R. Trusal,Albert W. Guzman,Carol J. Baker. Characterization of freeze-thaw induced ultrastructural damage to endothelial cells in vitro[J] 1984,In Vitro(4):353～364
  • 6晏菊芳,鲍朗,伍卫华,万波,李胜富.中国钩端螺旋体强毒株017膜蛋白基因的质粒载体构建及表达[J].中华微生物学和免疫学杂志,1999,19(2):117-120. 被引量：5

共引文献11

同被引文献12

  • 1Faine S. Leptospirosis. In: Evans A, et al. ed.Bacterial infections of humans. New York:Plenum Medical Book Company,1998,395-419.
  • 2Reng S, Fu G, Jiang X, et al.Unique physiological and pathogenic features in Leptospira interrogans reveals by whole-genome sequencing. Nature,2003, 422:888-893.
  • 3Sambrook J, Fritsch E F, Maniatis T. Molecular cloning: a laboratory manual. 2nd ed. New YorK : Cold Spring Harbor Laboratory Press,1989, 4.39-4.50,18.47-18.56.
  • 4Sara M , Sleytr U B. S-layer proteins. J Bacteriol,2000, 182(4):859-868.
  • 5Blaser M J , Gotschlich E C. Surface array protein of Campylobacter fetus: cloning and gene structure. J Biol Chem, 1990,265(24):14529-14535.
  • 6Tummuru M K R , Blaser M J. Rearrangement of sapA homologs with conserved and variable regions in Campylobacter fetus. Proc Natl Acad Sci USA,1993, 90:7265-7269.
  • 7Wang B, Kraig E , Kolodrubetz D. A new member of the S-layer protein family: characterization of the crs gene from Campylobacter rectus. Infect Immun, 1998, 66(4):1521-1526.
  • 8Evenberg D, Versluis R, Lugtenberg B. Biochemical and immuno-logical characterization of the cell surface of the fish pathogenic bacterium Aeromonas salmonicida . Biochem Biophys Acta, 1985, 815(2):233-244.
  • 9Haake D A, Champion C I, Martinich C, et al. Molecular cloning and sequence analysis of the gene encoding OmpL1, a transmembrane outer membrane protein of pathogenic leptospira spp.J Bacteriol, 1993,175(13):4225-4234.
  • 10Shang E S, Summers T A, Haake D A. Molecular cloning and sequence analysis of the gene encoding LipL41, a surface-exposed lipoprotein of pathogenic Leptospira species. Infect Immun,1996,64:2322-2330.

引证文献1

西南师范大学学报（自然科学版）
2004年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部