摘要
目的探讨辅助性T细胞17（Th17）和白细胞介素17（IL-17）在非小细胞肺癌（NSCLC）患者外周血中的表达和临床意义。方法选取原发性NSCLC患者40例为观察组,健康志愿者40例为对照组,采用流式细胞仪检测2组外周血Th17细胞水平,采用酶联免疫吸附法（ELISA）检测血清IL-17水平。比较2组外周血Th17细胞及IL-17水平,分析观察组患者外周围血Th17细胞及IL-17水平与临床病理特征的关系。结果观察组外周血中Th17细胞和IL-17水平明显高于对照组（P〈0.01）;鳞状细胞癌患者外周血Th17细胞水平和IL-17表达高于腺癌患者,且Ⅲ~Ⅳ期患者明显高于Ⅰ~Ⅱ期患者,差异均有统计学意义（P〈0.01）。结论在NSCLC的发生发展的过程中,Th17细胞及细胞因子IL-17可能发挥一定作用。
Objective To explore the expression and its significance of Th17 cell and IL-17 in peripheral blood of patients with non-small cell lung cancer（ NSCLC）. Methods Forty patients with primary NSCLC were enrolled and designed as observational group and 40 healthy volunteers as control group. Flow cytometry（ FCM） was used for detecting the level of Th17 cell of 2 groups,and enzyme linked immunosorbent assay（ ELISA） for detecting the level of IL-17 of 2 groups. The levels of Th17 cell and IL-17 cell in peripheral blood of patients were compared and their relationship with clinicopathological factors was analyzed. Results The levels of Th17 cell and IL-17 in peripheral blood were higher in observational group than in control group（ P〈0. 01）,higher in patients with squamous-cell carcinoma than in patients with adenocarcinoma,higher in Ⅲ ~ Ⅳpatients than in Ⅰ ~Ⅱ patients（ P〈0. 01）. Conclusion Th17 cell and IL-17 may play a certain role in the occurrence and development of NSCLS.
出处
《实用临床医药杂志》
CAS
2015年第23期34-36,共3页
Journal of Clinical Medicine in Practice
基金
中国高校医学期刊临床专项资金(11524298)
