摘要
背景:内皮细胞体外培养形成的单层内皮细胞能否抵抗血流的冲击,如何实现细胞均匀分布生长是实验中的常见问题。目的:针对背景问题,研制一种细胞培养装置,可提供细胞生长的三维环境,实现细胞贴硅胶弹性腔管壁生长,改善细胞的分布不均。方法:从控制单元、机械单元和细胞培养单元介绍了该装置的结构与功能,并利用该装置进行一例脐静脉内皮细胞培养实验。结果与结论:旋转式三维细胞培养装置为细胞代谢提供了一定条件,该装置培养的细胞能贴壁均匀,生长良好。
BACKGROUND： It is widely used that vitro culturing endothelial cells to study atherosclerosis mechanism,and the key issues are if the monolayer endothelial cells could resist blood attack and how to achieve cells growth well-distributed.OBJECTIVE： To develop a device that could supply a three-dimensional condition for cells to realize growth stick to elastic silicon chamber in order to improve growth uneven.METHODS： It introduced structure and functions of the device from controlled unit,mechanical unit and cells culture unit,and then used it to carry out an umbilical vein endothelial cells culture experiment.RESULTS AND CONCLUSION： Rotary three-dimensional cells culture device could provide condition for cells metabolism and growth sticking to elastic silicon chamber well-distributed.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第19期3531-3533,共3页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
上海理工大学医疗器械与食品学院微创励志创新基金
课题名称:三维内皮细胞培养装置研制与实验~~
