摘要
对采集的土壤样品中解钾菌进行分离与鉴定,再以荒漠沙为土壤基质,加入含菌量为0、0.16、0.48、0.80、1.20、1.60（×108/CFU·m L-1）的菌液进行发芽试验,研究了解钾菌对棉花种子的发芽率、幼苗的鲜重、芽长和根长、超氧化物歧化酶（SOD）、过氧化物酶（POD）和丙二醛（MDA）含量的影响。结果表明,分离出的解钾菌为胶质芽孢杆菌（Bacillus mucilaginosus）。当沙漠土壤中加入不同含菌量的菌液后：棉花种子发芽时间提前0∽24 h,发芽率随着菌含量的增加呈先增大后减少的趋势,当菌含量为0.8×108CFU·m L-1时发芽率最高为70.29%;茎的长度增加0.5∽1.2 cm,主根的长度缩短0.20∽0.65 cm,但促进了侧根的生长,幼苗的鲜重提高了0.0662∽0.1582 g;幼苗中的相关酶和丙二醛含量分别有不同程度的增加和减少。
The potassium bacteria obtained from collected soil samples were isolated and identified,and then the effects of different contents from viable bacteria on the cotton seed germination rate,fresh weight,shoot and root length of seeding,the content of superoxide dismutase（ SOD）,peroxidase（ POD） and malondialdehyde（ MDA）were studied using desert sands as soil matrixes. The results showed that the potassium bacteria was Bacillus mucilaginosus. When different bacterial contents in bacterial liquid were added into desert soil,germination time of cotton seeds was 0 ∽ 24 hours earlier than the control experiment. Germination rate showed a tendency to become increased first and then went decreased with the addition of bacterial content,reaching a highest value of 70. 29%when the bacterial content was 0.8×108CFU·m L-1. The length of the cotton stem and taproot were relatively increased by 0.5 ∽ 1.2 cm and shortened by 0.20 ∽ 0.65 cm,respectively. However,the growth of lateral root was promoted,and the fresh weight of seedlings was increased by about 0.0662 ∽ 0.1582 g. In the meantime,both the content of related enzymes and malondialdehyde in the seedlings became increased or decreased to some extent.
出处
《干旱地区农业研究》
CSCD
北大核心
2015年第6期77-82,共6页
Agricultural Research in the Arid Areas
基金
国家核设施退役及放射性废物治理科研重点项目(14zg6103)
国家农业科技成果转化资金项目(2012GB2G200469)
西南科技大学研究生创新基金资助(14ycx071)
