摘要
针对目前海参养殖中水产微生态制剂品种单一、施用效果不稳定等现状,作者从地瓜参(Acaudina molpadioidea)肠道分离土著肠杆菌(Enterobacter),筛选到1株具有较好拮抗作用的海洋细菌C-5,该菌对副溶血弧菌(Vibrio parahaemolyticus)、溶藻弧菌(Vibrio alginolyticus)、杀鲑气单胞菌(Aeromonas salmonicida)等7种细菌有拮抗作用;通过形态观察、生理生化和16S rDNA基因序列分析鉴定为霍肠杆菌(Enterobacter hormaechei);通过卤虫(Artemia)和药敏实验检测该菌具有安全性,其对卤虫的72 h半致死浓度(LC50)为108cfu/m L,只对20种常规抗生素中的5种产生耐药性;对C-5的发酵条件进行优化,该菌优化后的发酵条件为:温度30℃,pH 7.0,盐度2%。结果表明,C-5有望作为一种益生菌应用于海水养殖病害控制。
A probiotic strain of nativeEnterobacternamed as C-5 has been isolated from the intestine ofacaudina molpadioidea in this study. By observing colony morphology, analyzing physiological and biochemical characters, and sequencing of 16S rDNA, C-5 was identified asEnterobacter hormaechei. C-5 showed inhibitory activity against seven pathogens, such asVibrio parahaemolyticus,Vibrio alginolyticus,Aeromonas salmonicida. The secu-rity of C-5 was confirmed by theArtemiachallenge test and drug sensitivity tests. The LC50 of C-5 was found to be 108 cfu/mL, and it was resistant to five antibiotics among twenty used. The optimized fermentation condition of C-5 is： temperature 30℃ , pH 7.0, 2% salinity.Our results showed that C-5 could be used as a kind of probiotic applied to aquaculture disease control.
出处
《海洋科学》
CAS
CSCD
北大核心
2015年第10期29-35,共7页
Marine Sciences
基金
国家基金面上项目(31272697)
中国科学院知识创新工程重点项目(KZCX2-EW-Q215)
广东省省级科技计划项目(2014B030301064)
