摘要
对产胶原蛋白酶的一株沙雷氏菌DL-12进行紫外诱变处理,以明胶为底物,对诱变后的菌种进行初步筛选,通过鱼皮液化实验和发酵上清液中的胶原蛋白酶活力的比较,选出紫外诱变不同剂量下产生的产酶活力有明显提高的菌株DL-12(50s)-3,DL-12(50s)-4和DL-12(55s)-5,对这3种菌的产酶稳定性进行测定和比较,其中诱变菌DL-12(50s)-4发酵上清液中酶活力平均达98.37U/m L,比出发菌提高了43.54%,且具有稳定产酶特性。由于经紫外诱变获得的诱变菌DL-12(50s)-4高产胶原蛋白酶且产酶稳定,是具有应用前景的资源菌种。
Serratia entomophila DL-12 for producing collagenase was mutated by exposure to UV stimulation. Then, gelatin was used as substrate to perform preliminary screening of mutation by using fish skin liquefaction experiment and the activity of collagenase in the fermentation supernatant was compared. Three strains with higher producing enzyme ability of DL-12（50s）-3, DL-12（50s）-4 and DL-12（55s）-5 were screened out. The stabilities of enzyme production by three strains were compared. Among them, the collagenase activity produced by the mutated strain DL-12（50s）-4 was as high as 98.37 U/mL, which was 43.54% higher than that by the original. In addition, the enzyme production kept stable. The results indicated that after UV mutagenesis, the mutant of DL-12 （50s）-4 was a promising resource species owing to its stable high collagenase-producing performance.
出处
《工业微生物》
CAS
CSCD
2015年第6期43-47,共5页
Industrial Microbiology
基金
大连企业技术研发基金(No.221436)
