摘要
用含不同浓度丁酸钠完全培养液和无血清培养液培养分泌抗组织型纤溶酶原激活剂(t-PA)单克隆抗体的杂交瘤细胞。当丁酸钠浓度为0.25～0.5 mmol/L时,对杂交瘤细胞分泌抗体能力具有刺激作用,提高抗体效价2～4倍,对细胞生长无抑制作用。表明丁酸钠可作为一种添加剂用以提高杂交瘤细胞分泌抗体的能力。
Using different concentration of sodium butyrate in RPMI 1640-20 or serum-free medium to culture hybridoma cells which secreted monoclonal antibodies (McAbs) against tissue-type plasminogen activator (t-PA). The results indicate that 0.25mmol/L-0. 5mmol/L of sodium butyrate could incite hybridoma cells to produce McAbs and had no inhibition on hybridoma cell growth. The maximum titer of McAb was 1:2500, 2-4 fold more than control. Sodium butyrate may be as an additive to enhance the ability of hybridoma secreting McAbs.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1991年第6期346-348,共3页
Chinese Journal of Immunology
关键词
丁酸钠
抗体分泌
杂交瘤
细胞
Hybridoma culture
Sodium Butyrate
McAbs production
