摘要
通过试管法和玻片法检测抗B抗体的效价和亲和力研究了影响抗B杂交瘤细胞抗体分泌的部分因素 ,结果显示 :0 2 5mmol/L ,0 5mmol/L丁酸钠和部分浓度rhIL 6能明显提高抗B抗体的效价和亲和力 ;胰岛素和维甲酸不能提高抗体的效价和亲和力。HAT培养基重筛选可使部分小体积细胞迅速死亡 ,抗B抗体的效价和亲和力明显提高 ,提示丁酸钠 ,rhIL
Some influented elements of anti B antibody secreted from hybridoma was studied by testing the titer and affinity. The results showed that 0 25 mmol/L and 0 5 mmol/L sodium butyrate and various concentrations of rhIL 6 could significantly increase the titer and affinity of anti B. But it could not be promoted by insulin and retinoic acid. Some smaller ones of the experimental cells died rapidly in the HAT medium, after which the titer and affinity of anti B rose markedly. It is concluded the sodium butyrate and rhIL 6 could promote the titer and affinity of anti B secreted by hybridoma, surviving clones in HAT medium could secrete more anti B antibody.
出处
《药物生物技术》
CAS
CSCD
2000年第3期146-148,共3页
Pharmaceutical Biotechnology
