摘要
本文对生脉注射液中人参总皂甙含量测定的薄层扫描法进行了研究。探讨了共存组分干扰的排除。比较了薄层扫描法与薄层比色法回收试验,前者回收率为98.90±3.9%～99.42±5.9%;后者回收率为95.70±0.79%～96.20±0.99%,二者经t检验(t<t_(0.25)),无显著性差异。为生脉注射液质量控制提供了含量测定方法。
Shengmai injection is acompound pharmaceutical preparation ofpanax ginseng root. A sensitive TLC-de-nsitometric method for the determinationof ginseng saponins was described. It wasbased upon that total ginseng saponinswere extracted with n-butanol (saturatedwith water) from Shenmai injection, theextract was evaporated and diluted to asuitable volume with methanol. An aliq-uot was separated by TLC on a silica gelG plate, with chloroform-methanol-water(70:55:10) as the developer, and thenmeasurement with a Shimadzu CS-910dualwavelength thin layer chromatograp-hic scanner. Instrumental parameters are:reflection mode, zigzag scanning, λS=525nm, λR=780nm, Sx=3. Slit=1.25×1, 25mm, rate 20mm/min. The quantity poin-ted of ginseng saponin Re in the rangeof 1--4 ug was in good linear relationship.The recovery of ginseng saponins withTLC-densitometric method was 98.90±3.9%-99.42±5.9% and with TLC-colormetricmethod was 95.70±0.79%--96.20±0.97%.t-test showed that results obtained by bothmethods made no remarkable differenceto each other. This methods is sensitive,accurate and rapid. The internal qualityof Shenmai injection can be controlledwith this method.
出处
《华西药学杂志》
CAS
CSCD
北大核心
1989年第4期206-209,共4页
West China Journal of Pharmaceutical Sciences
关键词
生脉注射液
人参总皂甙
薄层扫描
Ginseng Saponins
Shenmai injection
TLC-Densitometry
