摘要
从2～3月龄北京鸭法氏囊得到的粗提物,经Se(?)hadex G-15和Molselect G-10两步分子筛层析后,得到了部分纯化的促PFC活性因子,该因子的分子量小于750dal。用高压液相色谱(HPLC)进一步分离活性组分,表明活性物质带有较多正电荷,极性较强。
Bursa of Fabricius is a very important organ for B-eell development. We sot bursa extracts from 2-3 months old Peking Duck. A partially purified PFC-stimulating active factor has been seperated from the extracts by twice molecular sieve chromatography, Sephadex G-15 and Moselect G-10. The M. W. of the active factor is below 750 dal. The results of HPLC analysis of the factor show that its polarity is relatively strong, and it has more positive charges.The works of purifying and looking for a new assay are still going on.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1991年第1期28-31,共4页
Chinese Journal of Immunology
基金
中国自然科学基金会85资助
中国自然科学基金会95号资助
关键词
法氏囊
B细胞
活性因子
分离
纯化
bursa of fabricius
B Cells
active factor
seperation
