摘要
为了探明α-蒎烯的2种衍生物3-甲氧基-4-羟基苯基-α-蒎烯马来酰亚胺基酰腙和(10z)-N-[5.7.7-三甲基(4.1.0)庚-4-乙二胺-3-亚基]苯胺对舞毒蛾(Lymantria dispar)3龄幼虫的毒力作用,采用饲料混药法研究了2种衍生物不同剂量处理下,其体内Car E、CAT、SOD和ACh E活性的变化规律。结果表明:2种衍生物对舞毒蛾3龄幼虫均有一定的毒力作用,在48 h时LC50分别为68.344、119.537 mg·kg-1,其中(10z)-N-[5.7.7-三甲基(4.1.0)庚-4-乙二胺-3-亚基]苯胺的毒性更加显著。3-甲氧基-4-羟基苯基-α-蒎烯马来酰亚胺基酰腙对舞毒蛾3龄幼虫体内Car E和ACh E活性有明显的抑制作用,最大抑制率为70.97%和71.23%,对CAT和SOD活性具有明显的激活作用,最大值分别为对照的1.62、1.42倍;(10z)-N-[5.7.7-三甲基(4.1.0)庚-4-乙二胺-3-亚基]对舞毒蛾3龄幼虫体内CAT、Car E和ACh E活性有明显的抑制作用,最大抑制率为67.4%、31.43%、26.04%,对SOD活性表现出先抑制后激活的现象。
In order to provide the concept and the actual reference to effectively control the harm of the gypsy moth and practice the concrete prevention and control work,we studied the effect of two α- pinene derivatives involving 3- methoxy- 4-hydroxyphenyl-α-pinene maleimide group acylhydrazone and（ 10z）-N-[5.7.7-trimethyl（ 4.1.0） heptyl- 4-ethylenediamine-3-subunit] aniline on the 3rd instar larvae of gypsy moth by using feed mixed drug law and measured the（ CarE）,（ CAT）,（ SOD） and（ AChE） activity by using enzyme activity assay. Both agents have a certain role in virulence on the3 rd instar larvae of gypsy moth,the LC50 were 68.344 and 119.537 mg / kg at 48 h,and（ 10z）-N-[5.7.7-trimethyl（ 4. 1. 0）heptyl-4-ethylenediamine-3-subunit] aniline have an more significant effect. When the 3-methoxy-4-hydroxyphenyl-α-pinene maleimide group acylhydrazone was dealt with the 3rd instar larvae of gypsy moth on the one hand its effects were more obvious on the CarE and AChE activity with the largest inhibition rate of 70.97% and 71.23%,respectively,and it had a clear activating effect on the CAT and SOD activity that the maximum values were 1.62 and 1.42 times than those of the control,respectively. When the（ 10z）-N-[5.7.7-trimethyl（ 4. 1. 0） heptyl- 4-ethylenediamine- 3-subunit] aniline was dealt with the 3rd instar larvae of gypsy moth,its effects were more obvious on CAT,CarE and AChE activity with the largest inhibition rate of 67.4%,31.43% and 26.04%,respectively,and it showed the inhibition first and activation later for SOD activity.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2015年第11期106-110,121,共6页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(30870394)
