摘要
目的研究HPV L1壳蛋白与P16蛋白联合运用在宫颈癌前病变诊断中的临床意义及对转归的预测价值。方法对208例TCT异常且HR-HPV阳性的患者进行宫颈活检并根据病理结果分组,同时检测其HPV L1壳蛋白和P16蛋白并根据两种抗体表达情况分为4组,并对其中随访到的155例患者进行HPV转阴分析。结果HPV L1壳蛋白和P16蛋白在不同级别宫颈组织中的表达均不同,CINⅠ组的L1壳蛋白阳性率高于其他CIN组(P<0.01),≥CINⅢ组的P16蛋白阳性率高于其他3组(P<0.01);随访病例中HPV L1壳蛋白(+)/P16蛋白(-)的转阴率高,尤其在CINⅠ患者中该组高于其他几组(P<0.05)。结论 HPV L1壳蛋白与P16蛋白联合检测可成为诊断及预测宫颈病变的一个有效指标。
Objective To evaluate the clinical significance of the combination detection of human papillomavirus（HPV）L1capsid protein and p16 protein in the diagnosis of cervical precancerous lesion and its predictive value to disease outcome.Methods 208 cases of abnormal in thinprep cytologic test and high risk（HR）-HPV positive were performed the cervical biopsy and grouped according to the histopathologic test.Meanwhile,these cases were detected the HPV L1 capsid protein and the p16 protein and then divided into 4groups according to the expression situation of these two kinds of antibody.Among them,155 followed up cases were performed the analysis of HPV negative conversion.Results The expression of L1 and P16was different among different grades of cervical tissues,the positive rate of L1 in the cervical intraepithelial neoplasia（CIN）Ⅰ group was higher than that of other CIN groups（P〈0.01）and the positive rate of P16 in the≥CINⅢ group was higher than that in the other 3groups（P〈0.01）.Among the follow-up cases,the negative conversion rate in the HPV L1（＋）/P16（-）was higher,especially which in the CINⅠgroup was higher than other groups（P〈0.05）.Conclusion The combined detection of HPV L1 capsid protein and P16 protein can be used as an effective indicator in the diagnosis and prediction of cervical lesions.
出处
《检验医学与临床》
CAS
2015年第22期3314-3317,共4页
Laboratory Medicine and Clinic
基金
重庆市卫生和计划生育委员会医学科研资助项目(20142170)
重庆市南岸区卫生和计划生育委员会医学科研资助项目
关键词
宫颈癌前病变
HPV
L1壳蛋白
P16
cervical intraepithelial neoplasia
HPV L1capsid protein
P16protein
