摘要
运用SSR分子标记技术,对来自相同或不同亲本的6个黑莓潜力杂交组合优株进行了分子鉴定,确定指纹图谱。结果表明,78对SSR引物中共有25对在4个亲本品种中具有多态性。将各自在不同杂交组合亲本中具有多态的引物用于优株鉴别,发现Kiowa×Arapaho组合中的3个优株有12对亲本间呈多态的引物可以鉴别,优株10-2n-1有6对扩增为杂合带型,10-2n-2和10-2n-5均有5对,其余则偏父本或母本一方,且有5对引物可以区分3个优株;来自Chester×Kiowa组合的优株10-5n-2在18对多态引物中多扩增为双亲重组类型,推测其性状可能多介于双亲之间;来自Hull×Kiowa组合的优株10-6n-1-1和10-6n-1在6对多态引物扩增结果中,10-6n-1-1多继承了父本的带型,而10-6n-1扩增多显示为双亲杂合带型。鉴定结果为育种中相同亲本杂交组合黑莓优株区分提供了依据,也一定程度上揭示了黑莓杂交重组中的复杂性和不确定性。
Using SSR molecular markers,DNA fingerprints of six potential hybrid strains from the same or different hybrid parents were identified. The results showed that 25 of the 78 pairs of SSR primers had polymorphism in the four parent varieties. After the polymorphic primers for parents of different hybrid combinations were further used for superior strain identification,for the hybrid combination of Kiowa × Arapaho,three superior strains could be identified by 12 SSR pairs which performed polymorphic between parents. The superior plant 10- 2n- 1 showed heterozygous bands by six pairs＇ amplification while 10- 2n- 2 and 10- 2n- 5 both had five pairs. The bands for the rest primers were all same to the father or mother and five pairs of primers could discriminate three superior strains. There was one superior plant 10- 5n- 2 selected from the hybrid combination of Chester × Kiowa,which could be identified from the parents by combination of 18 pairs of polymorphic primers. Thus it was speculated that the traits of 10- 5n- 2 might be between the parents. Superior plants 10- 6n- 1- 1 and 10- 6n- 1 were from the same hybrid combination Hull × Kiowa. By amplification of six pairs of SSR polymorphic primers,10- 6n- 1- 1 mainly inherited the male parent and 10- 6n- 1 tended to display parents＇ heterozygous bands. The identification of different strains of the same hybrid combination not only provided the basis for distinguishment between superior plants in future＇s blackberry breeding but also partly revealed the complexity and uncertainty in blackberry hybrid breeding.
出处
《江苏林业科技》
2015年第5期1-5,19,共6页
Journal of Jiangsu Forestry Science & Technology
基金
江苏省农业科技自主创新资金项目"早熟
无刺
小籽黑莓优良品种选育"(CX[14]2021)
中植农业科技创新基金"优质无刺早熟黑莓新品系筛选与展示"(201401)
关键词
黑莓
杂交优株
SSR标记
DNA指纹图谱
Blackberry（Rubus sp.）
Superior hybrid strain
SSR marker
DNA fingerprint
