摘要
目的检测Smad2在胃癌组织及正常胃组织的表达情况，探讨其与胃癌发生发展及临床病理学指标的关系。方法收集承德医学院附属医院2012—06—2012—12手术切除经病理证实为胃癌组织60例及正常胃组织30例。应用免疫组织化学法、蛋白质印迹法检测30例正常胃组织与60例胃癌组织Smad2蛋白的表达。结果60例胃癌组织中Smad2蛋白的阳性表达80％，30例正常胃组织标本中阳性率为47％，癌组织远低于正常胃组织组织中的表达，P〈O．05；Smadl蛋白的相对含量在30例正常胃组织为0．33±0．05，在60例胃癌组织为0．78±0．05，两者比较差异有统计学意义，P〈0．05。结论Smad2在胃癌中表达异常，Smad2蛋白的异常表达可能是胃癌发生的一个重要因素，并且与胃癌临床病理特征有着密切的关系。
OBJECTIVE To detect Smad2 expression in gastric cancer tissue and normal gastric tissue and explore its relationship with gastric cancer development and clinical pathology index. METHODS The immune histochemicai method and Western blotting technology to detect 30 cases of normal gastric tissue and 60 cases of gastric cancer tissue Smad2 protein expression. RESULTS Sixty cases of gastric cancer tissues Smad2 protein positive expression of 80%, positive rate was 47% in 30 cases of normal gastric tissue specimens, the expression of the cancer is far lower than the normal gastric tissue tissue （P〈0.05）; The relative content of Smadl protein in 30 cases of normal gastric tissue is 0. 33±0. 05, in 60 cases of gastric cancer tissue is 0.78±0.05, the more significant differences （P〈0.05）. CONCLUSION Smad2 abnormal expression in gastric cancer, Smad2 abnormal expression of protein may be an important factor of gastric cancer, and has close relation with the clinicopathological features of gastric cancer.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2015年第B22期36-37,48,共3页
Chinese Journal of Cancer Prevention and Treatment
