摘要
顺 二氯一氨 (4 氨基 2 ,2 ,6 ,6 四甲基哌啶 1 氧自由基 )合铂 (Ⅱ ) (简称HJ5 )是一种有研究与应用前景的新型铂抗癌配合物。建立了常规测定HJ5含量的高效液相色谱分析方法。流动相为V(乙腈 )∶V(水 ) =10∶90的溶液 ,检测波长为 2 10nm ,色谱柱为PhenomenexC18(15 0mm× 3 9mmi d ,10 μm) ;采用外标法测定了HJ5的线性方程、回收率和精密度。该方法能使HJ5峰与杂质峰很好的分离 ,其峰面积与检测量呈线性关系 ,最低检出限为 0 0 7μg。用 9g/L的氯化钠水溶液作溶剂 ,能有效抑制HJ5的水解 ,使其稳定时间达到
Cis dichloroamine (4 amino 2,2,6,6 tetramethylpiperidine 1 oxyl) platinum(Ⅱ)(abbreviated to HJ5) is a novel platinum anticancer compound which has prospects for research and application. In order to establish a conventional analytical method, an HPLC has been studied. Under the conditions of acetonitrile water (10∶90, volume ratio) as mobile phase, detection wavelength of 210 nm, a Phenomenex column C 18 (150 mm×3 9 mm i d ,10 μm). The linear equation, recovery and accuracy of the method were determined with external standard method. The HPLC has satisfactory resolution between the peaks of HJ5 and impurities. The peak areas of HJ5 were linear to its amounts detected and the detection limit was 0 07 μg. The hydration of HJ5 can be inhibited effectively by NaCl solution. In a 9 g/L NaCl solution, the stability can be kept for more than 2 hours which is enough for analysis.
出处
《色谱》
CAS
CSCD
北大核心
2002年第4期345-347,共3页
Chinese Journal of Chromatography
基金
云南省基金项目"含稳定氮氧自由基铂配合物 (Ⅱ )的合成及抗癌活性研究"(No :9815 0 17)
