摘要
目的建立高效液相色谱技术测定血浆中顺铂浓度的方法来进行药物代谢动力学的研究。方法用Waters色谱系统。色谱条件:2487双通道紫外检测器。C18色谱柱(大连依利特ODS2分析柱,250mm×4.6mm,5μm),外接菲罗门C18(4mm×4 mm保护柱)。恒温35℃,流速1.0ml·min-1,流动相水-甲醇(28∶72),Waters 1525泵,717自动取样器,进样量为20μl,Breeze色谱工作站。血浆中的顺铂经二乙基二硫代氨基甲酸钠衍生化后,用氯仿提取其产物Pt(DDTC)2,离心后直接取氯仿层进样,采用高效液相色谱仪在254 nm处测定药物浓度。结果本法能有效地测定兔血浆中的顺铂浓度。样品各组分分离良好,顺铂线性范围为0.20768-12.468 mg.L-1,相关系数r=0.9999,日间和日内变异系数均小于10%。结论该方法是一种高效、准确的检测方法,适用于顺铂药物动力学的研究。
Aim To develop an HPLC method for determination of the concentrations of the cisplatin （CDDP） in plasma of rabbits. Methods The Waters HPLC system was adopted, including a 1525 pump of Waters Company, a manual 717 injector, a variable wavelength detector, an Breeze Chem Station. The analytical column was an Hypersil ODS2 clumn （250 mm x 4. 6 mm, 5 μm） with an lichrosphere C18 guard column. The column tempterature was kept in 35 ℃, The mobile phase was water and methanol in the ratio of 28 ： 72. The flow rate was 1 ml · min^- l, the injected volumn of samples was 20 μl. Since CDDP could not be determined directly, the derivatization reaction with sodium diethyldithiocarbamate trihydrate （DDTC） was adopted. The plasma samples were added with 50 μl 5% DDTC （ in 0. 1 mol · L^-1sodium hydroxide solution）, mixed and incubated at 37 ℃ for 30 min. The samples were then extracted with chloroform. The chloroform layer was injected 20 μl and the variable wave- length dector detected the derivatives of CDDP at the wavelength of 254 nm. Result The method could detect effectively and ac- curately the concertrations of CDDP in plasma of rabbits. The different components of samples could be seperated effectively. The linear range of CDDP was 0. 20768 - 12. 468 mg · L^-1 （r = 0. 9999 ）. The RSD of intra-day and inter-day was 〈 10%. Conchmion It appears to be an accurate and effective method that fit for the Pharmacokinetic study by detecting the concentra- tion of CDDP in plasma of rabbits.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第5期692-695,共4页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No30672297)
广东省科技计划资助项目(No2006B35802002)
广州市科技计划资助项目(No2004Z3-D2091)
