摘要
通过置换反应水热合成法合成中空银铂纳米材料,并对其进行表征,利用它的催化活性制备了一种灵敏的标记型C-反应蛋白免疫传感器.传感器首先以中空石墨烯为固定材料将C-反应蛋白抗体固定在电极上,采用中空银铂纳米颗粒标记C-反应蛋白抗体,通过夹心法检测C-反应蛋白.在最优的实验条件下,对C-反应蛋白进行检测,其线性范围为0.1-150 ng/mL,线性相关系数为0.9643,检测限为0.02 ng/mL.
In this paper,silver platinum nanomaterials were synthesized and characterized through replacement hydrothermal synthesis. The catalytic activity of Ag / Pt nanomaterials to the reduction of H2O2 was utilized as a sensitive label to fabricate sandwich type of C- reaction protein immunosensor. Hollow graphene was used as immobilization matrix and hollow silver platinum nanomerials was labeled on the C- reactive protein antibody to construct sandwich C reactive protein immunesensor. Under the optimized experimental conditions,the detection range of the sensor is 0. 1—150 ng / mL with linear correlation coefficient of 0. 964 3 and the detection limits of 0.02 ng / mL.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
北大核心
2015年第6期888-895,共8页
Journal of Yunnan University(Natural Sciences Edition)
基金
国家自然科学基金(21465026
21165023)
