摘要
通过静电吸附作用将普鲁士蓝(PB)固定在玻碳电极(GCE)表面,再依次电沉积L-半胱氨酸(L-Cys)、氯金酸(HAuCl4).通过氯金酸与甲胎蛋白抗体(anti-AFP)中氨基的键合作用,将抗体固定在电极表面,最后用牛血清白蛋白(BSA)封闭电极表面的非特异性吸附位点,从而成功制备了一种新型的电流型甲胎蛋白免疫传感器.实验利用循环伏安法对电极的制备过程及性能进行了表征,结果表明该免疫传感器对AFP有很好的电流响应,其线性范围为0.01～200.0ng/mL,检出限为0.003ng/mL.该实验方法操作简便,制得传感器灵敏度高,实现了对AFP的定量分析.
First of all, a layer of Prussian blue is fixed on the surface of glassy carbon electrode （GCE）. And then L- Cys, HAuCl4are electrolytically deposited in turn. Through the bonding effect of HAuCl4 and an anti-AFP, the antibody will be fixed on the electrode surface. Finally, bovine serum albumin （BSA） was applied to block the non-specific adsorption sites of the immunosensor, thus a new AFP Immunosensor comes successfully into shape. The preparation process of the electrode and its performance are then subjected to characterization by cyclic voltammetry. The findings show： the said sensor exhibits a high response sensitivity to electric current with a linear range of 0.01-200.0 ng/mL and the detection limit of 0. 003 ng/mL. The method is easy to operate and the sensor thus made is highly sensitive, which makes the quantitative analysis of AFP a reality.
出处
《内江师范学院学报》
2012年第8期36-39,45,共5页
Journal of Neijiang Normal University
基金
四川省教育厅自然科学重点项目(20122A-84)
内江师范学院自然科学科研项目(12NJZ15)
