摘要
建立一种简便、快速的固相萃取-高效液相色谱（HPLC）测定13种复合维生素（注射用）中2,6-二叔丁基-4-甲基苯酚（BHT）的方法。色谱柱为Inertsil？ODS-SP,（5μm,4.6 mm×250 mm）,流动相为甲醇与水（体积比90∶10）,流速为1.0 m L/min,检测波长为280 nm,柱温为25℃。结果：BHT在0.5005-32.032μg/m L范围内具有良好线性关系,检测限为0.12μg/m L,定量限为0.30μg/m L;加标回收率为86.66%-92.48%,相应RSD为2.77%-3.89%。结论：本方法快速简便,准确可靠,可用于13种复合维生素（注射用）中BHT的质量控制。
A simple and rapid method for the determination of BHT in 13 vitamins for injection was developed using Solid Phase Extraction（SPE） combined with HPLC. Inertsil#174; ODS-SP,（5 μm, 4.6 mm×250 mm） was used as separation column at 25 ℃.The eluent was methanol-water（Volume ratio is 90 to 10） at flow rate of 1.0 m L/min and a UV detector was adjusted at 280 nm.Under this HPLC conditions,the results showed that there was a good liner relationship in the concentration range of 0.5005~32.032 μg/m L.The detection limit of BHT was 0.12 μg/m L and the quantitation limit was 0.30 μg/m L. The recoveries of BHT in 13 vitamins for injection were in the range of 86.66 %~92.48 %;with relative standrad deviation of 2.77 %~3.89 %.The method is simple,reliable and sensitive for fast determination of BHT in 13 vitamins for injection.
出处
《广东化工》
CAS
2015年第19期152-153,159,共3页
Guangdong Chemical Industry
