摘要
目的:由于目前保健品中核黄素检测常用的荧光分光光度法操作繁琐、精密度较差,所以建立并优化适用于复合维生素保健品核黄素含量检测的HPLC-荧光检测方法,并与荧光分光光度法进行比较。方法:建立使用Diamonsil C18色谱柱(150×4.6 mm,5μm),以1%醋酸溶液-甲醇(70:30)为流动相,以荧光检测器(激发波长:445 nm,发射波长:530 nm)进行检测的液相色谱方法。对七种保健品中的核黄素分别用两种方法测定,考察结果之间的差别。结论:HPLC法结果的精密度和标准曲线线性明显优于荧光分光光度法,且操作更为简便,更适于保健品中核黄素的测定。
Objective：To develop a method for determination of riboflavin in health protection products by HPLC with fluorimetric detection,and to compare with the fluorescence spectrophotometry method.Methods：The chromatographic conditions included a Diamonsil C18 column（150×4.6 mm,5 μm） with the mobile phase of methanol-1% acetic acid water（30：70,v/v） and fluorimetric detector at a wave 1ength of 445 nm for excitation and 530 nm for emission.Samples were analyzed by two methods.Conclusion：The HPLC method is more suitable for determination of riboflavin in health protection products.
出处
《中国卫生检验杂志》
CAS
2010年第5期1059-1061,共3页
Chinese Journal of Health Laboratory Technology
