摘要
目的应用聚合酶链反应（PCR）．变性梯度凝胶电泳（DGGE）技术，分析30对母子口腔唾液中菌群的相似性。方法选取3～5岁高龋、中龋和无龋儿童及其母亲各10对作为研究对象，取其非刺激性全唾液，分别提取细菌总DNA，进行16SrRNA的PCR扩增及DGGE分析。结果母子间口腔菌群组成具高度相似性。其中，无龋组母子间配对相似系数明显低于高龋组及中龋组（P〈0．05）；高龋儿童的母亲平均龋失补牙面数（DMFS）明显高于中龋和无龋儿童的母亲（P〈0．05）。结论母亲是儿童口腔菌群获得的主要来源。
Objective This study aimed to analyze the similarity of the oral bacteria community in 30 mother-child dyads via polymerase chain reaction（PCR）-based denaturing gradient gel electrophoresis. Methods Unstimulated saliva samples were obtained from 30 mother-child pairs： 10 in caries-susceptive group, l0 in caries-moderate group, and l0 in caries-free group. DNAs were extracted for PCR amplification and denaturing gradient gel electrophoresis analysis. Results The compositions of the oral bacteria community in mother and child have a high degree of similarity. The oral bacteria community of children matched that of their mothers. The dice similarity coefficient of mother-child in the caries- free group was more statistically significant compared with the two other groups（P〈0.05）. However, mothers of caries- susceptive children had significantly higher decay missing filling surface（DMFS） scores than mothers of caries-free and caries-moderate children（P〈0.05）. Conclusion Children obtained oral bacteria from their mothers.
出处
《国际口腔医学杂志》
CAS
北大核心
2015年第6期664-667,共4页
International Journal of Stomatology
关键词
唾液
口腔
细菌群落
变性梯度凝胶电泳
saliva
oral
bacteria community
denaturing gradient gel electrophoresis
