摘要
目的:应用PCR-DGGE技术对双胞胎儿童牙菌斑细菌群落组成进行初步分析。方法:牙菌斑样本取自6对双胞胎儿童,年龄3.5～5.5岁,共17份。分别提取细菌总DNA,PCR扩增16SrDNA V3可变区,产物经DGGE指纹图谱分析其组成。结果:DGGE图谱显示,无龋和患龋双胞胎儿童牙菌斑菌属分布存在差异。结论:PCR-DGGE技术可较直观、灵敏地反应牙菌斑菌属的组成情况。
Objective： To detect bacterial community in dental plaque of twin children by PCR-DGGE.Methods： A total of 17 samples were collected from 6 twins.PCR-based denaturing gradient gel electrophoresis surveys microbial diversity by displaying PCR-generated 16S rDNA V3 fragment that migrate at different distances,reflecting the differences sequence of the fragment.Result： DGGE fingerprint patterns of dental plaque samples showed variation among them.Conclusion： PCR-DGGE method is a sensitive and effective approach to resolve the microflora structure of dental plaque.
出处
《口腔医学研究》
CAS
CSCD
2012年第10期997-999,共3页
Journal of Oral Science Research
基金
国家自然科学基金资助项目(编号:81170954)
