摘要
目的研究APOE小鼠肺炎衣原体感染后,对其动脉粥样硬化的形成是否有影响,为探索动脉粥样硬化的有效预防治疗途径提供依据。方法 48只APOE小鼠分为感染+高脂组、高脂组、感染组和对照组,每组各12只,喂养20周,进行血清抗CP抗体和血脂水平检测,油红O染色检测小鼠主动脉斑块形成面积。结果感染+高脂组、高脂组、感染组白介素-1β(IL-1β)、肿瘤坏死因子(TNF-α)的含量明显高于对照组(P<0.01);感染+高脂组IL-1β、TNF-α的水平明显高于感染组和高脂组(P<0.05);感染肺炎衣原体促进动脉粥样硬化斑块形成;油红O染色,感染组比对照组斑块面积均增加了25.0%,差异有统计学意义(P<0.01),感染+高脂组比高脂组斑块面积增加了23.0%,差异有统计学意义(P<0.01)。结论肺炎衣原体感染与高脂诱导和加速高脂饮食,促进APOE小鼠动脉粥样硬化的形成。
OBJECTIVE To study the effect of Chlamydia pneumoniae （CP） infection on atherosclerosis in APOE mice ,in order to provide reference for effective therapy of atherosclerosis .METHODS Forty‐eight APOE mice were divided into 4 groups with 12 mice in each group ,including infection ＋ hyperlipidemia group ,hyperlipidemia group ,infection group and control group .Mice were fed for 20 weeks and tested for levels of CP antibody and blood lipid .When the mice were sacrificed at 20 weeks of age ,the index of atherosclerotic formation was measured by oil red O staining .RESULTS The levels of IL‐1β and TNF‐α were significantly higher after infection with C . pneumonia in infection ＋ hyperlipidemia group ,hyperlipidemia group and infection group ,in comparison with control group （P〈0 .01） .The levels of IL‐1β and TNF‐αin infection ＋ hyperlipidemia group were higher than those in hyperlipidemia group and infection group （P〈0 .05） .The infection of CP can improve atherosclerotic for‐mation .According to the oil red O staining ,the area of atherosclerotic formation in infection group increased by 25% larger than control group （P〈0 .01） ,and increased in infection ＋ hyperlipidemia group by 23% larger than hyperlipidemia group （P〈0 .01） .CONCLUSION These findings suggest that C .pneumoniae infection can improve atherosclerosis in APOE mice .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2015年第20期4630-4632,共3页
Chinese Journal of Nosocomiology
基金
武汉市科研基金资助项目(WX13C45)
