摘要
目的探讨维生素D3结合高脂饲料建立大鼠动脉粥样硬化模型的方法。方法健康雄性Wistar大鼠40只随机平均分为四组:对照组、模型1组、模型2组、模型3组。对照组喂常规饲料,模型组喂高脂饲料。模型1组在造模前腹腔注射维生素D370万IU/kg,分4次每隔两天注射;模型2组在造模前注射60万IU/kg,造模后第3、6、9周分别注射10万IU/kg;模型3组在造模前和造模后第3、6、9周分别注射60万IU/kg;喂养12周后比较各组动物体重、血脂和主动脉病理变化。结果 12周后,模型组各组体重较对照组明显下降(P<0.01),其中模型3组体重下降最显著且死亡率最高;模型组各组的血清总胆固醇和低密度脂蛋白胆固醇与对照组比较均明显升高(P<0.01);模型1、2、3组造模成功只数分别为3只、8只和5只。结论高脂饲料结合造模前腹腔注射维生素D360万IU/kg,造模后第3、6、9周各补充10万IU/kg是比较有效的制备动脉粥样硬化大鼠模型方法。
Aim To explore an efficient method to establish a rat model of atherosclerosis using vitamin D3 （ VitD3 ） and atherogenic diet. Methods Forty healthy Wistar rats were randomly divided into 4 groups, ie, a control group receiving standard diet, three model groups receiving atherogenic diet for 12 weeks. At the same time, model group 1 were injected VitD3 7.0 × 105 IU/kg every two days before feeding for four times ; model group 2 were injected VitD3 6. 0 × 105 IU/kg before feeding and 1.0 × 105 IU/kg in the third, sixth and ninth week respectively after feeding; model group 3 were injected VitD3 6. 0 ×105 IU/kg before feeding and in the third, sixth and ninth week respectively after feeding. The body weights of all rats were recorded between 12 weeks. After 12 weeks, total cholesterol（ TC）, high-density lipoprotein cholesterol （ HDLC）, low-density lipoprotein cholesterol （ LDLC ）, and triacylglycerol （TG） were measured. HE staining was used to verify the atherosclerotic lesion in aorta. Results Compared with the control group, the body weights of model groups were seriously decreased （ P 〈 0. 01 ） , especially model group 3 and its high death rate. The serum levels of TC and LDLC in model groups were obviously increased than the control group. The numbers in three model groups pro- ducing typical atherosclerotic lesion in aorta were 3, 8 and 5 respectively. Conclusions It was an effective method for establishing a rat model of atherosclerosis by feeding atherogenic diet and injecting VitD3 , and the suitable dose of VitD3 was 6 ×10s IU/kg before feeding and 1.0 × 105 IU/kg in the third, sixth and ninth week respectively after feeding.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2012年第11期995-998,共4页
Chinese Journal of Arteriosclerosis
基金
南京医科大学基础医学院优势学科科研创新配套经费(JX10131801095)
