摘要
目的探讨外周代谢型谷氨酸受体5(m Glu R5)在颞下颌关节炎性疼痛中的作用。方法 SD大鼠36只,随机均分为6组,正常对照组(不做任何处理),阴性对照组(右侧颞下颌关节内注射50μl生理盐水),完全弗氏佐剂组(CFA组,右侧颞下颌关节内50μl CFA注射),MPEP-1组(1mm 2-甲基-6苯基乙炔嘧啶+50μl CFA),MPEP-2组(2.5mm MPEP+CFA),MPEP-3组(5mm MPEP+CFA)。将CFA注入右侧颞下颌关节腔内,诱导大鼠颞下颌关节疼痛。24h后检测各组大鼠的机械疼痛阈值,用Western-blot法和RT-PCR方法检测各组大鼠三叉神经节内m Glu R5和ERK1/2的蛋白和m RNA表达变化。结果 CFA组大鼠摆头阈值较正常组和阴性对照组明显下降(<0.01),MPEP-3组明显高于CFA组(<0.05)。三叉神经节内m Gu Rl 5和ERK1/2 m RNA及蛋白表达水平CFA组明显高于正常对照组和阴性对照组(<0.05),MPEP-3组明显低于CFA组(<0.05)。结论 m Glu R5参与颞下颌关节炎性疼痛,可能与ERK1/2信号通路相关。
Objective To study the expression of metabtrophic glutamate receptor subtype 5（mGluR5） in the trigeminal ganglion of rat with temporomandibular joint（TMJ） pain induced by CFA. Methods 36 rats were randomly divided into six groups averagely： normal control group （untreated）, negative control group（0.9%NaCl injection）, CFA group, MPEP-1 group （CFA ＋ lmM MPEP）, MPEP-2 group （CFA ＋2.5 mM MPEP）, and MPEP-3 group （CFA ＋ 5 mM MPEP）. The head withdrawal threshold was measured 24h after injection of CFA into the TMJ. Western-blot and RT-PCR were used to detect the expressions of mGluR5 and p-ERK1/2 protein and mRNA respectively. Results The head withdrawal threshold was significandy decreased in all CFA-treated groups compared with the control groups（P〈0.05）, but significantly increased in 5 mM MPEP group than in CFA group（P〈0.05）.The expression levels of mGluR5 and ERK1/2 protein and mRNA in the trigeminal ganglion showed a marked increase in CFA group than in control groups（P 〈0.05）, but decreased in 5 mM MPEP than in CFA group（P〈0.05）. Conclusion The involvement of mGluR5 in the temporomandibular joint pain might be related to ERK1/2 signal pathway.
出处
《解剖科学进展》
CAS
2015年第5期527-530,共4页
Progress of Anatomical Sciences
基金
辽宁省自然科学基金(No.2013021094)
