摘要
尿苷二磷酸葡萄糖脱氢酶(UGD)是多糖合成中的一个关键酶,广泛参与植物生长发育与非生物胁迫响应过程。采用生物信息学方法,在全基因组水平预测出10个大豆Gm UGD基因;序列分析结果显示,Gm UGD基因均具有UDP-葡萄糖/GDP-甘露糖脱氢酶家族的3个典型结构域;基因复制分析表明,80%Gm UGD基因存在复制事件;系统发生分析结果显示,Gm UGD基因分为三类,分类结果与基因复制分析结果基本吻合;基于Uni Gene数据库与GEO数据库的表达模式分析显示,根中Gm UGD基因的ESTs最多,上胚轴中最少;另外,Gm UGD基因能够响应盐碱胁迫诱导,在大豆根和叶中表达模式不同。结果可为进一步研究大豆Gm UGD基因功能提供理论依据。
UDP-glucose 6-dehydrogenase（UGD） is the key enzyme of polysaccharide biosynthesis, which plays important roles in the development and response to abiotic stresses of plant. A genome-wide analysis was conducted using bioinformatics and ten GmUGD genes of soybean were identified. Sequence analysis showed that all the GmUGD genes had three conserved domains which were typical in UDP-glucose/GDP-mannose dehydrogenase family. Gene duplication analysis indicated eighty percent of GmUGD genes were duplicated. Phylogenetic analysis showed that GmUGD genes were classified into three clusters, and the result was similar with gene duplication analysis. Expression pattern analysis which based on UniGene and GEO database indicated that more ESTs of GmUGDs were found in root than other tissues and none were found in epicotyls. In addition, the GmUGD genes responded to salinity-alkalinity stress and showed differential expression pattern under salinity-alkalinity stress. These results provide theoretical basis for further research of GmUGD genes function in soybean.
出处
《东北农业大学学报》
CAS
CSCD
北大核心
2015年第9期23-29,共7页
Journal of Northeast Agricultural University
基金
国家自然科学基金项目(31171578)
转基因生物新品种培育重大专项(2011ZX08004-002)
黑龙江省高校科技创新团队建设计划(2011TD055)
