摘要
TBP是真核生物的通用转录因子,在转录过程中扮演着重要的角色。采用生物信息学方法鉴定了家猪TBP蛋白家族成员,对家猪TBP蛋白进行染色体定位、理化性质和系统进化分析,并对其二级和三级结构进行预测。结果显示,家猪TBP蛋白家族包含3个成员,染色体定位发现其中2个TBP基因分布在第1染色体上,另1个位于骨架上;3个TBP蛋白家族成员的氨基酸数目分别为188、273、376,其中1个为疏水性蛋白、2个为亲水性蛋白;TBP蛋白二级结构以α-螺旋和无规则卷曲为主要组成部分,其家族成员的三级结构基本相似。
TBP（TATA binding protein） is a general kind of important transcriptional factors in eu- karyotes. This article analyzed the phylogenesis of TBP protein family sequences and the localization of TBP genome by using bioinformatics method and then predicted and analyzed their amino acid eomposition,physicochemical property,as well as secondary and tertiary structures. The predicted results of the TBP protein family of Sus scrofa showed that it consisted of three members. Further genetic mapping of TBP genome localization found that 2 TBP genes distributed on chromosome 1 and another located in a scaffold. The number of amino acid and hydrophobic quality of amino acid sequences in TBP protein families presented some differences. However, the predictive results of the secondary structure found that the main composition of 3 amino acid sequences was random coil,and the tertiary structure of the 3 amino acid sequences was similar.
出处
《河南农业科学》
CSCD
北大核心
2014年第3期128-132,共5页
Journal of Henan Agricultural Sciences
