摘要
目的：检测和分析健康成人外周血中γδT细胞的T细胞受体（ TCR）分子Vδ1~8亚群分布情况。方法：采集16名健康成人外周血，荧光定量反转录聚合酶链式反应检测其γδT细胞TCR分子Vδ1~8亚群的基因相对表达量，计算各亚群所占百分比。结果：健康成人外周血γδT细胞TCR分子Vδ1~8亚群的比例分别为：Vδ1（7.19±0.33）%、Vδ2（54.27±7.9）%、Vδ3（20.23±0.03）%、Vδ4（3.80±0.02）%、Vδ5（2.08±0.02）%、Vδ6（0.81±0.25）%、Vδ7（7.21±0.02）%、Vδ8（4.41±0.04）%。 Vδ2亚群均明显高于其他亚群（P〈0.01）。结论：我国健康成年人外周血γδT细胞TCR分子Vδ1~8亚群分布由多到少依次为Vδ2〉Vδ3〉Vδ1〉Vδ8〉Vδ7〉Vδ4〉Vδ5〉Vδ6。
Objective：To observe the distribution of Vδ1 to 8 subsets of the T cell receptor（TCR） molecule in the γδT cell of the human peripheral blood using real-time PCR. Methods：The mRNA expressions of the Vδ1 to 8 subsets of TCR molecule in theγδT cell in 16 healthy people peripheral blood were detected by real-time PCR,the respective percentage of whose were calculated. Results：The ratio of Vδ1,2,3,4,5,6,7 and 8 subsets were（7. 19 ± 0. 33）%,（54. 27 ± 7. 9）%,（20. 23 ± 0. 03）%,（3. 80 ± 0. 02）%,（2. 08 ± 0. 02）%,（0. 81 ± 0. 25）%,（7. 21 ± 0. 02）% and（4. 41 ± 0. 04）%,respectively. The ratio of Vδ2 was significantly higher than that in other subsets（P〈0. 05）. Conclusions：The distribution sequence from most to least of Vδ1 to 8 subsets of the TCR molecule in theγδT cell of the healthy people peripheral blood is Vδ2〉Vδ3〉Vδ1〉δV8〉Vδ7〉Vδ4〉Vδ5〉Vδ6.
出处
《蚌埠医学院学报》
CAS
2015年第9期1148-1151,共4页
Journal of Bengbu Medical College
