摘要
本文首次应用抗骨形成蛋白单克隆抗体(BMP-McAb)研究了骨形成蛋白(BMP)在骨纤维异常增殖症及骨化纤维瘤中的细胞定位。ABC 法染色结果显示,在骨纤维异常增殖症中,BMP 主要分布于纤维组织中,纤维母细胞、纤维细胞及胶原纤维均有较强的阳性着色,骨组织周围一些胞体较大的细胞含有丰富的 BMP,多数纤维性骨组织呈阳性染色;在骨化纤维瘤中,纤维组织多为弱阳性染色,只有骨组织周围的骨母细胞为阳性着色。实验结果进一步证实这两种病变中的骨组织的生长方式不同。此外,运用这种染色方法有助于对这两种疾病的临床病理鉴别诊断。首次发现骨纤维异常增殖症内含有丰富的 BMP,进一步证实了 BMP 与机体部分骨疾患有着较为密切的关系。
The monoclonal antibody against bone morphogenetic protein(BMP-McAb)was firstused for demonstration of bone morphogenetic protein(BMP)in fibrous dysplasia of boneand ossifying fibroma.In fibrous dysplasia of bone,avidn-biotin complex method(ABC)demonstrated that BMP mainly existed in the fibrocellular tissue,fibroblasts,mesenchymalcells,and collagen which were all strong positive.Some large cells around bone tissuecontained rich BMP in cell plasma.A large part of the fibro-bone(woven bone)wasstained positively.In ossifying fibroma,fibrous tissues showed weakly positive,and onlythe osteoblasts around the bone rim showed positive.The results demonstrated that therewere different types of bone growth between the two different bone diseases.In addition,fibrous dysplasia and ossifying fibroma could be differentiated in clinical pathologicaldiagnosis by this staining method.The finding of fibrous dysplasia's containing rich BMPIndicated that BMP may have close relation with other bone diseases.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
1989年第4期198-200,共3页
West China Journal of Stomatology
