摘要
目的观察雷米普利抑制肾素-血管紧张素系统(RAS)阻断后对糖尿病(DM)大鼠胰岛再生衍生因子3α(Reg3α)表达的影响。方法 SD大鼠10只采用链脲菌素40mg/kg腹腔注射建立DM模型:B组5只为模型对照(用生理盐水灌胃10周);C组5只采用雷米普利50mg·kg-1·d-1灌胃10周。另取5只大鼠腹腔注射等量柠檬酸缓冲液作为空白组(A组,用生理盐水灌胃10周)。基因芯片检测大鼠胰岛相关基因表达;实时定量PCR和Western blot验证相关基因mRNA和蛋白表达。结果 B组Reg3α表达较A组上调7.9倍,C组较B组Reg3α表达上调7.1倍。C组胰岛染色面积和胰岛形态结构较B组增加和改善。结论雷米普利抑制胰岛局部RAS系统,促进DM大鼠的胰岛再生;其作用可能部分通过影响Reg3α基因的表达实现。
Objective To observe the effect of blocking renin-angiotensin system（RAS） by ramipril on the expression of regenerating islet-derived 3 alpha（Reg3a） in diabetic rats. Methods The diabetic model was induced by intraperitoneal injection of streptozotocin 40 mg/kg in 10 rats, of which 5 rats （group C） were treated with ramipril 50 nag · kg-1· d-1 by lavage for 10 weeks and 5 rats （group B） were taken as model controls（treated with normal saline by lavage for 10 weeks）. Another 5 ST） rats（group A） by intraperitoneal injection in the same volume of citrate butter were taken as blank controls （treated with normal saline by lavage for 10 weeks）. The expressions of rat islet-related genes were detected by gene chip. qRT-PCR and Western blot were used to verify the mRNA and protein expressions of related genes. Results Compared to group A,Reg3a expression in group B was upregulated by 7. 9 times. Compared to group B, Reg3a expression in group C was upregulated by 7. 1 times. The area of islet dyeing was more and islet morphology structure was improved in group C than those in group B. Conclusion Ramipril may inhibit local RAS of pancreatic islet and promote islet regeneration of DM rats, which may be partially via influencing Reg3a gene expression.
出处
《江苏医药》
CAS
2015年第17期1999-2001,F0003,共4页
Jiangsu Medical Journal
基金
江苏省南通市科技局基金(K2009017)
