摘要
目的:研究银杏叶提取物(GBE)对胃癌SGC7901细胞增殖与凋亡的影响。方法:0、5、10、20、50、100、200μg/ml GBE培养细胞48 h后,MTT法测定细胞活力并计算抑制率。0、10、50、200μg/ml GBE培养细胞48 h后,流式细胞仪检测细胞周期分布及凋亡率。结果:5、10、20、50、100、200μg/ml GBE培养细胞48 h后,对细胞生长有明显抑制作用,且呈剂量依赖关系。10、50、200μg/ml GBE培养细胞48 h后细胞凋亡率升高。结论:GBE可一定程度上抑制SGC7901细胞增殖,诱导其凋亡,以前者为主。
OBJECTIVE：To investigate the effects of Ginkgo biloba extract（GBE） on proliferation and apoptosis of gastric cancer cell line SGC7901. METHODS：After the cells were cultured in 0,5,10,20,50,100 and 200 μg/ml GBE for 48 h,cell viability was determined by MTT method and inhibition rate was calculated. After the cells were cultured in 0,10,50 and 200μg/ml GBE for 48 h,flow cytometry was used to detect cell cycle distribution and the apoptosis rate. RESULTS：Following 48 h culture of cells in 5,10,20,50,100 and 200 μg/ml GBE,the growth of cells were obviously inhibited in a dose- dependent manner. After 48 h culture of cells in 10,50 and 200 μg/ml GBE,the apoptosis rate was increased. CONCLUSIONS：GBE can inhibition the proliferation of SGC7901,and induces it apoptosis,the former dominated.
出处
《中国药房》
CAS
北大核心
2015年第25期3510-3512,共3页
China Pharmacy
关键词
银杏叶提取物
胃癌SGC7901细胞
增殖
凋亡
Ginkgo biloba extract
Gastric cancer cell line SGC7901
Proliferation
Apoptosis
