摘要
单核细胞增生性李斯特菌(Lm)是一种重要的食源性人兽共患病病原菌,所引起的李斯特菌病虽然发病率低,但病死率高。传统的Lm分型方法步骤繁琐、重复性差、分辨率低。目前,多种针对Lm的分子分型方法逐渐完善,而且分辨力、分辨率、操作性和敏感性均优于传统分型方法。就目前Lm分子流行病学研究中常用的分子分型方法(PFGE分型、MLVA分型、MLST分型、MVLST分型、Lineage分型)阐述、比较,为Lm食源性疾病监测、暴发的诊断及溯源提供参考。
Listeria monocytogenes （Lm） is a gram-positive, facultative intracellular bacterium which is widely distributed in the environment, and can infect more than 40 kinds of animal. Lm is described as an important foodborne pathogen that can cause zoonotic listeriosis, with the characteristics of low morbidity and high mortality. Traditional typing methods for Lm are complex, poor repetitiveness and low-resolution. Currently, more and more molecular typing methods for discrimination of Lm are being established, they become more sensitive and discriminative compared with the phenotypic subtyping methods and are widely used for typing Listeria isolates. In this study, five common molecular typing methods （PFGE, MLVA, MLST, MV- LST, Lineage） are introduced and compared, providing valuable reference for tracing the source of pathogenic Lm contamina- tion and transmission routes.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2015年第8期757-762,共6页
Chinese Journal of Zoonoses
基金
国家自然科学基金(No.31472193)
江苏省科技支撑计划(No.BE2012367)
江苏省自然科学基金(No.BK2011446)联合资助~~
