摘要
目的 :应用 Alarm a blue测定法评价中药复方软肝煎对 HSC/ T6增殖的影响。方法 :实验分为小牛血清、大鼠血清及药物血清 3组 ,各组再分为 5 %、10 %及 2 0 % 3个浓度组 ,每组 4只大鼠。利用中药血清药理学 ,通过MTT法和 Alarma blue测定法测定软肝煎药物血清影响 HSC/ T6增殖的情况。结果 :在 MTT测定法中 ,与小牛血清组相比 ,大鼠血清组的 5 %浓度差异有显著性意义 (P <0 .0 5 ) ,10 %、2 0 %浓度差异有非常显著性意义 (P <0 .0 1) ,药物血清组各浓度差异均有非常显著性意义 (P <0 .0 1) ;与大鼠血清组相比 ,药物血清组的 5 %、10 %浓度差异有显著性意义 (P <0 .0 5 ) ,2 0 %浓度差异有非常显著性意义 (P <0 .0 1)。在 Alarm a blue测定法中 ,2 4 h时 ,与小牛血清组比较 ,大鼠血清组与药物血清组的 10 %浓度差异均有非常显著性意义 (P <0 .0 1) ,与大鼠血清组相比 ,药物血清组的 10 %浓度差异有显著性意义 (P<0 .0 5 ) ;30 h时 ,与小牛血清组比较 ,大鼠血清组的 10 %浓度及药物血清组的 10 %浓度差异均有显著性意义 (P<0 .0 5 ,<0 .0 1) ,与大鼠血清组相比 ,药物血清组的 10 %浓度差异有非常显著性意义 (P <0 .0 1)。结论 :软肝煎能抑制 HSC/ T6的增殖 ,具有抗肝纤维化作用 ;Alarma blue测定法是一种?
Objective:To evaluate the effect of serum containing Ruanganjian decoction on the proliferation of HSC/T6 by alarma blue assay.Methods:All of the sera were divided into 3 groups: calf serum group, rat serum group and drug-containing serum group. Each group was subdivided into three subgroups of 5 %, 10 % and 20 % (n=4 each). MTT assay and alarma blue assay were used to measure the effect of Ruanganjian decoction on the proliferation of HSC/T6.Results: In the groups assayed with MTT, rat serum of 5 % could inhibit HSC/T6 proliferation with more remarkable effect than calf serum (P< 0.05), while rat sera of 10 % and 20 % with very significant difference (P < 0.01); There was significant difference among different subgroups of drug-containing serum (P< 0.01); As compared with rat serum, drug-containing sera of 5 % and 10 % could inhibit HSC/T6 proliferation with remarkable difference (P< 0.05), while serum of 20 % with very significant difference (P< 0.01). In the groups assayed with alamar blue, as compared with calf serum, rat and drug-containing sera of 10 % could inhibit HSC/T6 proliferation after 24 h with more significant difference (P< 0.01); As compared with rat serum, drug-containing serum of 10 % could inhibit HSC/T6 proliferation with statistical difference (P< 0.05); As compared with calf serum, rat serum of 10 % could inhibit HSC/T6 proliferation after 30 h with significant difference (P< 0.05), while drug-containing serum of 10 % with more significant difference (P< 0.01); As compared with rat serum, drug-containig serum of 10 % could inhibit HSC/T6 proliferation after 30 h with very significant difference (P< 0.01). Conclusion: (1) Serum containing Ruanganjian decoction could inhibit the proliferation of HSC/T6, antagonize hepatic fibrosis. (2) Alarma blue assay was a creditable method. (3) Different animal sera had different effects on HSC proliferation and it was preferable to use a single animal serum in cultured medium.
出处
《中国中西医结合消化杂志》
CAS
2002年第4期219-222,共4页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
