摘要
目的研究合成产物聚乙二醇单甲醚接枝壳聚糖(m PEG-g-CS)中聚乙二醇接枝率对其纳米胶束自组装特性的影响,为自组装纳米胶束作为药物载体的研究提供理论依据。方法按5种不同的壳聚糖(CS)与聚乙二醇单甲醚(m PEG)的投料质量比(CS/m PEG分别为1∶1、1∶3、1∶6、1∶9、1∶12),通过甲醛连接法合成不同接枝率的m PEG-g-CS。采用稳态荧光探针法荧光分光光度计测定其荧光强度计算m PEG-g-CS临界胶束浓度,并通过粒径分析仪分析自组装纳米胶束粒径分布。比较接枝率不同的聚合物对其临界胶束浓度及粒径大小的影响。结果 CS质量一定的情况下,随着m PEG投料质量比增加(CS/m PEG分别为1∶1、1∶3、1∶6、1∶9),聚合物接枝率和临界胶束浓度均依次增高(P<0.05)。其中投料质量比为1∶1组自组装纳米胶束平均粒径最大,投料比为1∶6组平均粒径最小;除投料比为1∶9组与1∶12组平均粒径比较差异无统计学意义(P>0.05)以外,其余各组平均粒径比较差异均有统计学意义(P<0.05)。CS/m PEG 1∶1组和1∶3组粒径分布出现双峰,1∶6组、1∶9组、1∶12组粒径分布呈现均一单峰分布。结论通过控制m PEG和CS的投料质量比可获得不同接枝率的m PEG-g-CS样品,m PEG-g-CS能自组装成纳米胶束,且接枝率不同的聚合物其纳米胶束自组装特性和粒径分布亦出现差异。根据其胶束的稳定性和粒径分布,投料比为1∶6和1∶9的聚合物有望作为纳米药物载体。
Objective To research the effect of grafting degree on characteristic behavior of self assemble monomethoxy poly（ ethylene glyco1）-grafted-chitosan nano micelle,thereby providing theoretical basis for the self assembled nano micelle as drug delivery systems. Methods Different grafting rates of m PEG-g-CS products were synthesized by controlling the m PEG /CS feed mass ratio（ CS / m PEG 1∶1,1∶3,1∶6,1∶9,1∶12） through formaldehyde linking method. The critical micellar concentration of m PEG-g-CS was determined by measuring the flurescence intensity of the pyrene as fluorescent probe,and the size distribution of self-assembled nano micelle particle was analyzed by dynamic laser light scateering. The effect of grafting degree on characteristic behavior of self assemble monomethoxy poly（ ethylene glyco1）-grafted-chitosan nano micelle was compared.Results Within a certain range,the grafting ratio and the critical micellar concentration of m PEG-g-CS increased as the weight ratio between m PEG and CS increasing（ CS / m PEG 1∶1,1∶3,1∶6 and 1∶9）,and the differences were significant（ P〈0. 05）. The m PEG-g-CS（ CS/m PEG 1∶1） nano micelle had a maximum particle diameter while the m PEG-g-CS（ CS/m PEG 1∶6） nano micelle had a minimum particle diameter. The differences among the other groups were statistically significant differences（ P〈0. 05） except for the difference between CS / m PEG 1 ∶9 and CS / m PEG 1 ∶12（ P〈0. 05）. For CS /m PEG 1∶1 and 1∶3,the particle size distribution result showed bimodal distribution,and for group CS / m PEG 1 ∶6,1 ∶9and 1∶12,it showed uniform particle size distribution. Conclusions Different grafting rates of m PEG-g-CS samples can be synthesized by controlling the m PEG / CS feed mass ratio in a certain range,and the self-assembly characteristic behavior and particle size distribution of nano micelle are differed significantly. According to the results of the micelle stability and particle size distribution,m PEG-g-CS（ CS / m PEG 1∶6 and 1∶9） are promising to be nano-drug delivery material.
出处
《新乡医学院学报》
CAS
2015年第7期599-602,共4页
Journal of Xinxiang Medical University
基金
国家自然科学基金资助项目(编号:U1304819
81401519)
研究生科研创新支持计划资助项目(编号:YJSCX20414Z)
国家级大学生创新训练计划项目(编号:201310472034
201410472030)
关键词
聚乙二醇
壳聚糖
胶体滴定
临界胶束浓度
纳米胶束
polyethylene glycol
chitosan
colloid titration
critical micellar concentration
nano micelle
