摘要
目的 :研究中国沈阳地区儿童变形链球菌群细菌 (Mutansstreptococci,MS)的母子传播情况。方法 :选取 16对母子的唾液 ,在选择培养基上培养后计数MS的菌落数量 ,用随机引物聚合酶链反应 (arbitrarilyprimedpolymerasechainreaction ,AP_PCR)鉴定其基因型。结果 :儿童口腔中 5 6 5 %的MS基因型与母亲一致 ;而且母亲与儿童唾液MS计数水平呈正相关关系。结论 :沈阳地区儿童口腔MS主要来源于母亲。
Objective:The aim of this study was to investigate mother_to_child transmission of Mutans streptococci in Shenyang area of China.Methods: Saliva samples of 16 mothers and their children were collected and cultured on selective medium for Mutans streptococci. Arbitrarily primed polymerase chain reactions (AP_PCR) with randomly chosen primers were performed to genotype these isolates.Results: The PCR results demonstrated that Mutans streptococci in 54 2% of the mother/child pairs were the same in genotypes. Similar genotypes in mothers and children were found significantly more frequently in mothers who had more than one genotype. Significant correlation was found in colony forming units (CFU) between mothers and their children.Conclusion: This suggests that Mutans streptococci could be transmitted from mothers to their children. Significant correlation was found between the number of genotypes of Mutans streptococci in mothers and the transmission of this group of streptococci from mothers to their children.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2002年第4期268-270,共3页
West China Journal of Stomatology
关键词
沈阳地区
变形链球菌
聚合酶链反应
母子传播
龋齿
儿童
Mutans streptococci\ \ arbitrarily primed polymerase chain reaction\ \ mother_to_child transmission
