摘要
石斛的药用成分含量直接影响其品质，筛选高品质的药用石斛对其生产和应用具有重要意义。采用热水浸提法和酸性染料比色法比较研究了26种野生石斛和9个春石斛品种成熟茎段多糖和生物碱含量的差异。野生种和春石斛品种多糖含量分别在2．0％～39．1％和9．4％～23．7％之间；而生物碱含量则分别在0％-0．638％和0．156％-0．308％之间。肿节石斛、铁皮石斛和齿瓣石斛在所有样品中多糖含量较高，而报春石斛的生物碱含量则显著高于其他石斛，其次为兜唇石斛、玫瑰石斛和金钗石斛。粼中国植物志》石斛分组，检测的14种为石斛组，平均多糖和生物碱含量均较高，分别达23．2％和0．2％，其次为春石斛品种，分别达18．4％和0。2％。不同石斛的多糖和生物碱含量差异较大，生产应用中需根据需要选取合适的石斛。
The quality of Dendrobium species is depended on the contents of medicinal compositions, and selecting high quality Dendrobium is important for production and application. The polysaccharide and alkaloid contents were determined among 35 Dendrobium wild species and cuhivars using the hot water extraction and acid dye colormetric method, respectively. The results showed that the polysaccharide contents of Dendrobium wild species and cultivars were between 2.0%-39.1% and 9.4%-23.7%, respectively, and the alkaloid contents of those were 0%-0.638% and 0.156%-0.308%, respectively. D.pendulum, D.officinale and D.devonianum had higher polysaccharide contents than others, while the alkaloid content of D.primulinum was significantly higher than that of others, followed by D. aphyllum, D.crepidatum and D.nobile. Meanwhile, Sect. Dendribium had the highest average polysaccharide content （23.2%） and alkaloid content （0.2%） in all samples, followed by cultivars （18.4% and 0.2%）. The polysaccharide and alkaloid contents had significant differences among different Dendrobium wild species and cultivars, and selecting the proper Dendrobium should be in accord with objectives in practice.
出处
《中国农学通报》
2015年第24期242-246,共5页
Chinese Agricultural Science Bulletin
基金
广东省科技计划项目"我国药用石斛种质资源鉴评
创新利用与产品研发"(2013B090600147)
国家青年科学基金"基于SLAF-seq技术的石斛高密度遗传图谱构建及生物碱含量QTL定位"(31400597)
中央农业科技推广专项资金"观赏兼药用石斛新品种的种苗繁育与示范推广"(粤财农〔2012〕480号)
关键词
野生石斛
春石斛品种
多糖含量
生物碱含量
Dendrobium wild species
Dendrobium cultivars
polysaccharide content
alkaloid content
