摘要
为了探明八角金盘灰斑病的病原菌种类,便于对其进行有效防治,以从云南、重庆和贵州等地收集的灰斑病标样为研究材料,通过分离、纯化,采用形态学结合分子系统学分析的方法,进行病原菌的鉴定,并在此基础上进行防治药剂室内筛选研究。结果表明:引起八角金盘灰斑病的病原菌为中央叶点霉;10%苯醚甲环唑水分散粒剂、50%多菌灵可湿性粉剂和10%多抗霉素粉剂等3种杀菌剂对该菌有较强的抑制作用,其EC50分别为1.9298、2.1805和6.0194 mg/L;80%代森锰锌可湿性粉剂和6%春雷霉素可湿性粉剂对该菌的抑制效果较差,其EC50分别为103.2997和161.1234 mg/L。
The samples of grey spot disease collected from Yunnan, Chongqing and Guizhon were identified by isolation, purification, mor- phology and molecular systematics analysis, and then the fungicides to control pathogen of Fatsia japonica grey spot disease were screened in a laboratory to probe pathogenic species of Fatsia japon/ca grey spot disease and its effective control by fungicides. The results showed that the pathogen to cause atsia japonica grey spot disease is Phyllosticta capitalotsis. 10 % Difenoconsde WDG, 50 % Carbendazim WP and 10 % Polyoxins have the strong inhibition effect against pathogen to cause Fatsia japonica grey spot disease and EC50 of 10 % Difenoconszle WDG, 50 % Carbendazim WP and 10 % Pelyoxing is 1. 9298, 2.1805 and 6.0194 mg/L, respectively. ECho of 80% Manccceb WP and 6 % Chunleimeisu WP is 103.2997 and 161.1234 mg/L separately, which indicates 80 % Mancozeb WP and 6 % Chunleimeisu WP have weak inhibition effect against pathogen of Fatsia japonica grey spot disease.
出处
《西南农业学报》
CSCD
北大核心
2015年第3期1130-1134,共5页
Southwest China Journal of Agricultural Sciences
基金
科技基础性工作专项"西南喀斯特地区特色微生物资源及多样性调查"(2014FY120100)
关键词
八角金盘灰斑病
病原菌
鉴定
杀菌剂
筛选
Fatsia japonica grey spot
Pathogen
Identification
Fungicide
Screening