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不同驯化手段下多氯联苯降解菌群落结构差异分析

Analysis on the Community Structure of PCBs Degrading Bacteria under Different Domestication Means
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摘要 [目的]研究不同驯化手段下多氯联苯降解菌群落结构差异,为多氯联苯污染场址的生态修复提供借鉴。[方法]应用PCRDGGE技术对8种驯化手段下的活性污泥进行分析。[结果]加入同一种PCB单品的体系中,生物群落的相似度相对较高;好氧体系之间的相似性较好氧与厌氧体系之间的相似性要高;在好氧条件下,高氯PCB体系较低氯PCB体系中生物相丰富;好氧体系较厌氧体系的生物相丰富;一些物种在好氧体系和厌氧体系中均存在。[结论]该研究有利于多氯联苯降解菌库的充实和完善。 [Objective] The aim was to analyze the differences of community structure of PCBs degrading bacteria under different means of domestication means,and provide references for the ecological restoration of contaminated sites by polychlorinated biphenyls. [Method] PCRDGGE technology was used to analyze the activated sludges under 8 kinds of domesticated methods. [Result] To join the same kind of PCB item system,the similarity of biological communities was relatively high; In aerobic system the similarity was higher than between aerobic and anaerobic systems; Under aerobic conditions,biological phase in the high chlorine PCB system was richer than in low chloride PCB system;biological phase in aerobic system was recher than the anaerobic system; Some species was exsited in aerobic system and anaerobic systems.[Conclusion]Some empirical rules were obtained,which was conductive to the enrichment and improvement of the library of PCBs degradation bacteria.
作者 梁文涓 闫勇
出处 《安徽农业科学》 CAS 2015年第22期11-13,共3页 Journal of Anhui Agricultural Sciences
关键词 多氯联苯 PCR-DGGE 活性污泥 群落结构 Polychlorinated biphenyls PCR-DGGE Activated sludge Community structure
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