摘要
目的:建立被动皮肤过敏性试验中准确且具可重复的阳性对照。方法:30只SD雄性大鼠随机分为1个阴性对照组(氯化钠注射液+弗氏不完全佐剂)和4个阳性处理组(阳性1组:卵白蛋白,阳性2组:卵白蛋白+百白破疫苗,阳性3组:卵白蛋白+弗氏不完全佐剂,阳性4组:卵白蛋白+弗氏完全佐剂及弗氏不完全佐剂),分别致敏5次制备抗血清,通过皮内注射被动致敏、48 h后激发,以皮内蓝斑有无及直径判定结果。结果:阴性对照组和阳性1组、2组均未见蓝斑,阳性3组、4组出现蓝斑,其1∶2和1∶8注射点蓝斑直径均大于5 mm。结论:被动皮肤过敏性试验中,致敏时结合使用弗氏佐剂可使阳性率达到100%,以保证试验的准确性和可重复性。
Objective： To establish an accurate and repeatable method for positive control of passive cutaneous anaphylaxis （PCA） test. Methods： 30 SD rats were randomly divided into 1 negative control group（ sodium chloride injection plus Freund＇s adjuvant incomplete） and 4 positive treatment groups （the positive 1 group： ovalbumin, the positive 2 group： ovalbumin plus diphtheria-pertussis-tetanus vaccine, the positive 3 group： ovalbumin plus Freund＇s adjuvant incomplete, the positive 4 group： ovalbumin plus Freund＇s adjuvant complete and Freund＇s adjuvant incomplete）were set in the experiment. Firstly, antiserum was prepared with 5 subcutaneous injections. Secondly, passive sensitization was conducted by intradermal injection of antiserum. After 48h, the immuno-stimulation was fol- lowed by intravenous injection of equal amount of antigen. Finally, the result was evaluated by inspecting the amount and diameter of intradermal blue spots. Results：The blue spot was not found in the negative control group, the positive 1 group and the positive 2 group. While both the positive 3 group and the positive 4 group produced intradermal blue spots. The average diameter of blue spots of the 1 ： 2 and 1： 8 antiserum dilution injection was larger than 5 mm. Conclusion： In PCA test, by using different sensitive treatment, we found that sensitization with Freund＇s adjuvant could carry out 100% positive results. It is an accurate and reliable method for establishment of positive control in PCA test.
出处
《中国药物评价》
2015年第3期129-131,共3页
Chinese Journal of Drug Evaluation
关键词
被动皮肤过敏性试验
阳性对照
佐剂
蓝斑
Passive cutaneous anaphylaxis test
Positive control
Adjuvant
Blue spot
