摘要
目的:探讨大鼠皮肤被动过敏试验(PCA)的影响因素,为选择敏感试验条件和提高试验预测的准确性提供依据。方法:采用卵白蛋白和牛血清白蛋白为PCA试验的抗原,在不同条件下(加与不加佐剂、致敏的途径、大鼠的品系、制备抗血清的最佳取血时间、卵白蛋白与牛血清白蛋白在免疫原性上的差异等)进行PCA试验。结果:①加用佐剂能够显著增强SD和Wistar大鼠机体对免疫应答的敏感性。②ip致敏和背部皮下致敏均可获得良好的PCA反应,但ip致敏加用佐剂后会诱发大鼠腹腔慢性炎症。③SD大鼠与Wistar大鼠在抗原免疫应答的敏感性方面基本相似,但在同一试验中采用不同品系的大鼠进行PCA试验优于用单一品系的大鼠。④大鼠在致敏后16 d抗体中IgE含量高于致敏后14 d。⑤卵白蛋白在PCA试验中的免疫原性优于牛血清白蛋白。
Objective：To investigate the factors of passive cutaneous anaphylaxis（PCA） in rats to select experimental conditions and improve the accuracy of forecasts for experiments.Method：Egg albumin and bovine serum albumin were selected as test antigens for the PCA.PCA experiments were carried out in different experimental conditions（with or without kasa agents,allergens means,rat strains,the optimal time for preparing antiserum of blood withdrawn,the difference in immunogenicity of egg albumin and bovine serum albumin, etc. ）. Resuit：（1） Adjuvant could significantly enhance the sensitivity Intraperitoneally and subcutaneously injection sensitization of immune response, in SD and Wistar tast. （2） both could get good response of PCA, but intraperitoneally injection with adjuvant could induce chronic inflammation of abdominal cavity. （3） Sensitivity of immune response to antigen was similar in SD and Wistar rats. However, to use different strains of rats with PCA were better than to use single stain of experimental rats. （4） Content of IgE in serum after 16 days sensitization was higher than that in 14 days sensitization. （5） Egg albumin was superior to bovine serum albumin in immunogenicity of PCA test.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第18期160-162,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家"重大新药创制"科技重大专项(2008ZX09202-009)
