摘要
建立了薄层色谱法检测中药中的48种非法染色色素。分别采用乙腈、0.1%甲酸甲醇溶液、甲醇∶0.1%甲酸（3∶2）混合溶液超声提取样品粗粉。将乙腈提取液与脂溶性色素对照溶液点于同一硅胶G板上,以石油醚∶乙醚∶无水甲酸（80∶20∶1）为展开剂展开。将0.1%甲酸甲醇溶液提取液与水溶性碱性色素对照溶液点于同一硅胶G板上,以乙酸乙酯∶乙醇∶水∶氨水（12∶1∶1∶1.2）为展开剂展开。将甲醇∶0.1%甲酸（3∶2）提取液与水溶性酸性色素对照溶液点于同一硅胶G板上,以乙酸乙酯∶乙醇∶水∶氨水（6∶4∶2∶0.2）为展开剂展开。均在日光下检视。48种色素的检测限为0.025～0.6μg。本法快速简便,可应用于中药材多色素染色初步筛查。
A TLC method was established for the simultaneous qualitative analysis of 48 banned pigments in traditional Chinese medicine. Sample powders were extracted by ultrasonication with acetonitrile, 0.1% formic methanol solution and methanol ： 0.1% formic acid （3：2）, respectively. The acetonitrile extraction and the liposoluble pigments reference solution were applied to a silica gel plate separately and developed with petroleum ether ：diethyl ether ： formic acid （80 ： 20 ： 1）. The 0.1% formic methanol solution extraction and the basic pigments reference solution were developed with ethyl acetate ： ethanol ： water ： amonia （12 ： 1 ： 1 ： 1.2）, and the methanol ： 0.1% formic acid （3： 2） extraction and the acid pigments reference solution were developed with ethyl acetate ： ethanol ： water ： amonia （6 ： 4 ： 2 ： 0.2）. The plates were observed under daylight. The limits of detection of 48 banned pigments were 0.025 - 0.6 μg. This method, with simple pretreatment and rapid analysis, was suitable for the simultaneous scan of pigments in traditional Chinese medicine.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2015年第7期695-700,共6页
Chinese Journal of Pharmaceuticals
基金
国家药典委员会中国药典2015年版附录色素测定法研究项目的资助
关键词
色素
中药
薄层色谱
检测
pigment
traditional Chinese medicine
TLC
detection
