摘要
对发酵液中纳他霉素的色谱分离条件进行了优化,分别探讨了流动相的组成、冰醋酸水溶液与甲醇的比例、流动相中冰醋酸浓度对保留时间的影响,确定了纳他霉素的分离条件。该方法在5.0 mg/L～300 mg/L范围内呈良好的线性关系,相关系数r^2=0.999 8。纳他霉素的加标回收率在100.07%～102.41%之间。相对标准偏差(KSD)小于1.56%(n=3)。最低检测限为0.25μg/mL(S/N=3)。用甲醇超声提取样品,并通过优化的色谱条件对其进行分析,测定了发酵液中纳他霉素的含量。
t A rerversed --Phase high Performance liquid chormtaography （RP-HPLC） method for the determination of natamycin in fermentation borth was developed. The separation conditions of the chromatography were optimized with a Eclipes XDB C18 （5 μm, 4.6 mm× 150 mm） separation column. The relationship of acetic acid concentration in mobile Phase and the retention time, the effect of mobile Phase composition and the ratio of acetic acid solution and methanol on retention time were studied separately. Quantitative results showed that the limits of detection were 0.25 μg/mL for the natamycin in fermentation borth .The linear range for the quantification of the natamycin was 5.0 mg/L-300 mg/L and the coefficient （r2）was 0.999 8. The spiked recoveries from natamycin samples were 100.07%-102.41%, and their relative standard deviations were less than 1.56% （n=3）.
出处
《食品工业》
北大核心
2008年第2期66-69,共4页
The Food Industry
基金
沈阳农业大学中青年硕士导师科研基金(200503)
关键词
反相高效液相色谱
流动相
冰醋酸
发酵液
纳他霉素
high perofrmnace liquid chromatography （HPLC）
mobile Phase
glacial acetic acid
fermentation broth
natamycin
